Monolayer assemblies of several phthalocyanine derivatives, e.g. copper tetrakis(n-butoxycarbonyl)phthalocyanine, showing highly anisotropic absorption spectra have been obtained.

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Combined multilayers of purple membrane and octa-n-butoxyphthalocyanine as a pH sensitive dye were prepared by horizontal lifting method. The photo-induced proton-pumping activity of bacteriorhodopsin was observed by change of absorption spectra of the multilayers in water, and the one-way transport of proton was confirmed.