Spatiotemporal environmental changes lead to disturbances in wild plant habitats, particularly in regions characterized by changeable land use and cover. The present study aims to characterize wild plant habitats in the River Nile region of Dakahlia Governorate using a multidisciplinary approach, incorporating remote sensing, GIS and sampling analyses. Twenty-four stands representing the wild plant habitats in the River Nile region were geographically identified, sampled and analyzed. Water and soil samples were investigated for physical and chemical characteristics. Two calibrated multispectral Landsat images dated 1999 and 2019 were processed to produce LULC, NDSI, NDMI and NDSI to characterize wild plan habitats. The floristic composition showed the presence of 64 species belonging to 53 genera and 28 families. Ecologically, the recorded plant species in the present work can be classified into four main categories, which are separated into three communities according to the TWINSPAN classification. Results showed that the annual loss of agricultural lands (3.98 km2) is closely relevant to the annual expansion of urban areas (4.24 km2). Although the uncontrolled urban sprawl caused loss of agricultural lands, it leads to the expansion of wild plant habitats, represented mainly by the sparse class and partially by the moderately dense class as obtained from NDVI. The increase in mean values of the moisture (NDMI) from 0.034 in 1999 to 0.64 in 2019 may have arisen from the increase in total areas of wild plant habitats during the investigated period (1999–2019). This might increase the suitability of conditions for wild habitats which induces the proliferation of natural plants.

Artemisia judaica L. (Shih Balady, family Asteraceae), is a medicinal plant that grows in the desert, commonly used as tea by population in Egypt Sinai and of Saudi Arabia. The present study aims to evaluate antimicrobial and antioxidant activities of aerial parts of A. judaica collected from the inland desert (Wadi Hagoul) of Egypt. Total phenolics, tannins, alkaloids, flavonoids, and saponins were measured in the plant of interest. According to the reduction of DPPH the anti-oxidant activity was measured. Different extracts of A. judaica were screened for their antimicrobial activity against nine pathogenic microorganisms using filter paper disc assay. The secondary metabolites of A. judaica were examined and proved that it was rich in tannins and phenolics (13.29 and 7.62 mg/g dw). The IC50 values of A. judaica extract was 1.78 mg.ml-1 compared to standard catechol (0.15 mg.ml-1). In the present study, the extracts of A.  judaica showed unusual activity depending on the type of bacteria and fungi. Also, ethyl acetate and methyl alcohol extracts showed considerable broad spectrum (77.78%, each) against both bacteria and fungi, followed by acetone, then methylene chloride and petroleum ether extracts. This study showed that A. judaica extracts could be used as antimicrobial substances in pharmaceutical and food preservation systems.

Environmental and land-use changes put severe pressure on wild plant habitats. The present study aims to assess the biodiversity of wild plant habitats and the associated spatiotemporal environmental changes in the coastal region of Dakahlia Governorate following an integrated approach of remote sensing, GIS, and samples analysis. Thirty-seven stands were spatially identified and studied to represent the different habitats of wild plants in the Deltaic Mediterranean coastline region. Physical and chemical characteristics of soil samples were examined, while TWINSPAN classification was used to identify plant communities. Two free Landsat images (TM and OLI) acquired in 1999 and 2019 were processed to assess changes via the production of land use and cover maps (LULC). Moreover, NDSI, NDMI, and NDSI indices were used to identify wild plant habitats. The floristic composition indicated the existence of 57 species, belonging to 51 genera of 20 families. The largest families were Asteraceae, Poaceae, and Chenopodiaceae. The classification of vegetation led to the identification of four groups. Canonical Correspondence Analysis (CCA) revealed that electrical conductivity, cations, organic carbon, porosity, chlorides, and bicarbonates are the most effective soil variables influencing vegetation. The results of the spectral analysis indicated an annual coverage of bare lands (3.56 km2), which is strongly related to the annual increase in vegetation (1.91 km2), water bodies (1.22 km2), and urban areas (0.43 km2). The expansion of urban and agricultural regions subsequently increased water bodies and caused occupancy of bare land, resulting in the development of wild plant habitats, which are mostly represented by the sparse vegetation class as evaluated by NDVI. The increase in mean moisture values (NDMI) from 0.03 in 1999 to 0.15 in 2019 might be explained by the increase in total areas of wild plant habitats throughout the study period (1999–2019). This may improve the adequacy of environments for wild habitats, causing natural plant proliferation.

Euphorbia retusa (family Euphorbiaceae) is an annual plant in the desert of Egypt with a 20 to 60 cm high. The aerial part of the plant containing latex is used in many biological activities. This study aimed to determine the phytochemical constituents, antioxidant, antimicrobial properties and allelopathic activities of aerial parts of E. retusa collected from Wadi Arabah, North Eastern Desert, Egypt. Standard methods were used for the qualitative and quantitative of tannins, saponins, flavonoids, alkaloid, phenolics, steroids, terpenoids, and glycosides. The antioxidant activity was measured based on the reduction of DPPH.Antimicrobial activity was evaluated against pathogenic bacteria, as well as the allelopathic potential against Chenopodium murale was assayed. E. retusa attained the highest values of saponins, tannins, and phenolics compared. In the DPPH test system, the IC50 value of the antioxidant inhibition for E. retusa was 802.74µg mL-1. The data obtained indicated that, the sensitivity of the seedling growth to the extract was higher than the germination of the test species. In addition, the root growth of C. murale was more affected than the shoot system to the inhibitory allelopathic activity induced by E. retusa. At higher concentration (40 g L-1) the germination of the tested seed was highly inhibited by 51.77%, while the shoot and root were reduced by 60.98% and 74.55%, respectively. However, the opposite response was observed at the lower concentration (2.5 g L-1).In conclusion, theextract of E. retusa plant has phytotoxic properties and thus contains phytotoxic substances