A rapid and sensitive method based on ultrafast liquid chromatography–tandem mass spectrometry was developed and validated for simultaneous determination of Sudan I, Sudan II, Sudan III, and Sudan IV levels in rat whole blood. Cleanert C18 mixed-mode polymeric sorbent was used for effective solid-phase extraction cleanup. Separation was carried out on a reversed-phase C18 column (100 mm×2.1 mm, 1.8 μm) using 0.1% (v/v) formic acid in water/0.1% (v/v) formic acid in acetonitrile as the mobile phase in gradient elution. Quantification was performed by an electrospray ionization source in the positive multiple reaction monitoring mode using D5-Sudan I as the internal standard. Calibration curves showed good linearity between 0.2 and 20.0 μg/L, with correlation coefficients higher than 0.9990. The average recovery rates were between 93.05% and 114.98%. The intra- and inter-day relative standard deviations were within 6.2%. The lower limit of quantification was 0.2 μg/L. All the analytes were found to be stable in a series of stability studies. The proposed method was successfully applied to a pharmacokinetic study of four Sudan dyes after oral administration to rats.

We observed the level of lowering blood pressure (BP) and heart rate (HR) of SHR by total falconoid from Jumi. We used SHR models, intervene them by total falconoid from Jumi, and rats were divided into SHR control groups, nifedipine control group, total falconoid high dose groups, total falconoid medium dose groups and total falconoid low dose groups. We observed the level of Bp and HR by non-invasive blood pressure. Total falconoid from Jumi significantly reduced the levels of Bp and HR when after 3 h treating by single dose test. Total falconoid from Jumi reduced the levels of them and lasted four hours and it took slower effect than nifedipine in successive administration. Effect of lowering Bp and HR among three dose groups of total falconoid wasn't significant difference. Total flavonoids from Jumi could reduce Bp and HR of SHR, and it had a better long-term effect for mild-to-moderate hypertension.