For different tasks, probe tip should be changed in the 3D vision coordinate measuring system and the accurate determination of probe tip center position is critical. A novel and simple approach for calibrating the probe tip center position of the light pen is presented in this paper. Hundreds of images of the light pen with different postures are collected while the probe tip is kept in firm contact with a reference conical hole. The probe tip position is determined by computing the rotation matrix and translation vector from the obtained images by using the least square fitting method. The experimental results demonstrate the effectiveness of the proposed approach. Its repeatability reaches 0.033 mm, 0.030 mm, and 0.043 mm in x, y, and z axes, respectively, and its convergence speed is satisfactory.
The pharmacokinetic (PK) properties of enrofloxacin (ENR) and its metabolite ciprofloxacin (CIP) were investigated in crucian carp following oral administration at different dose levels (5, 10, 20, 40 mg/kg body weight). The disposition kinetics of ENR was found to be linear over the dose range studied. Serum half-lives ranged from 64.56 to 72.68 hr. The in vitro and ex vivo activities of ENR in serum against a pathogenic strain of Aeromonas hydrophila were determined. In vitro and ex vivo bactericidal activity of ENR was concentration dependent. Dosing of 10 mg/kg resulted in an AUC/minimum inhibitory concentration (MIC) ratio of 368.92 hr and a C /MIC ratio of 7.23, respectively, against A. hydrophila 147 (MIC = 0.48 μg/ml), indicating a likely high level of effectiveness in clinical infections caused by A. hydrophila with MIC value ≤ 0.48 μg/ml. Modeling of ex vivo growth inhibition data to the sigmoid Emax equation provided the values of AUC /MIC required to produce bacteriostasis, bactericidal activity, and elimination of bacteria, these values were 21.70, 53.01, and 125.21 hr, respectively. These findings in conjunction with MIC data suggested that ENR at the dose of 7.81 mg/kg predicted a positive clinical outcome and minimize the risk of emergence of resistance.
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