Increasing evidence demonstrate that circular RNAs (circRNAs) play critical role in regulation of gene expression, which participate in the pathogenesis of cancer, including chronic myeloid leukemia (CML). In this study, we aimed to investigate the expression profiling of circHIPK3 in CML. We found that circHIPK3 was significantly upregulated in peripheral blood mononuclear cells (PBMC) and serum samples from CML compared with healthy controls. High circHIPK3 expression predicted a poor outcome of CML patients. Further loss-function experiments suggested the oncogenic role of circHIPK3 in CML. Our findings provide insights on the role of circHIPK3 in the development and treatment of CML.

Well-dispersed nanocomposites based on Ca-montmorillonite decorated by porphyrin-functionalized titanium dioxide (H2TCPP–TiO2–MMT) were synthesized by a facile strategy. H2TCPP–TiO2–MMT was demonstrated to possess the intrinsic peroxidase-like activity and could catalyze 3′,3′,5′,5′-tetramethylbenzidine (TMB) oxidized by H2O2 accompanied with the color change from colorless to blue in a short time under visible light irradiation. The optimum experimental conditions, robustness, as well as Michaelis–Menten kinetics were investigated. The fluorescent probe assay indicated that the good performance of H2TCPP–TiO2–MMT was attributed to the •OH radicals from H2O2 decomposition, which was induced by the electron–hole pairs of H2TCPP–TiO2–MMT generated under visible light irradiation. Based on its peroxidase activity, a convenient colorimetric sensor for detecting glutathione (GSH) was proposed. The detection limit for GSH was 0.057 μM, with the linear detection range of 0.1–20 μM. Notably, besides the enhanced peroxidase-like activity, another merit for this sensor was that the reaction time can be controlled accurately by adjusting irradiation time compared to traditional colorimetric sensors, which would benefit the accuracy in detecting GSH. In addition, results of anti-interference and detecting GSH in human serum assays indicated that the H2TCPP–TiO2–MMT was predicted to be one of the promising materials for fast visual colorimetry.

The effects of C-phycocyanin (C-PC) on atherosclerosis and the regulatory effects of CD59 gene on anti-atherosclerotic roles of C-PC were investigated. Apolipoprotein E knockout (ApoE(−/−)) mice were randomly divided into four groups: control group, C-PC treatment group, CD59 transfection group and C-PC+CD59 synergy group. The mice were fed with high-fat-diet and treated with drug intervention at the same time. Results showed the atherosclerotic mouse model was successfully established. CD59 was over-expressed in blood and tissue cells. Single CD59 or C-PC could reduce blood lipid levels and promote the expression of anti-apoptotic Bcl-2 but inhibit pro-apoptotic Fas proteins in endothelial cells. The expression levels of cell cycle protein D1 (Cyclin D1) and mRNA levels of cyclin dependent protein kinase 4 (CDK4) in smooth muscle cells were restrained by CD59 and C-PC. CD59 or C-PC alone could inhibit the formation of atherosclerotic plaque by suppressing MMP-2 protein expression. In addition, C-PC could promote CD59 expression. So both CD59 and C-PC could inhibit the progress of atherosclerosis, and the anti-atherosclerotic effects of C-PC might be fulfilled by promoting CD59 expression, preventing smooth muscle cell proliferation and the apoptosis of endothelial cells, reducing blood fat levels, and at last inhibiting the development of atherosclerosis.

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