A toluene-degrading bacterium, Acinetobacter sp. Tol 5, shows noteworthy adhesiveness mediated by two types of cell appendages. In this study, we obtained a less-adhesive mutant, T1, which lost both types of appendages, and investigated how the cell appendages affect the adhesion properties of this useful bacterium for environmental technology. Wild-type cells attained irreversible adhesion to polyurethane carriers within 30 s, while adhesion of T1 cells was still reversible at that time. While T1 showed decreased adhesion with decreasing ionic strength and did not adhere at all at 0.015 mM, adhesion of the wild type was fully independent of ionic strength. Acinetobacter sp. Tol 5 was also found to be not motile. Our results suggest that through the long distant interaction mediated by the appendages between the cells and surfaces, Tol 5 cells can attain irreversible adhesion very quickly without approaching the vicinity of the substratum.

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The bonding performance of a surface pre-reacted glass ionomer (S-PRG) filler-containing self-adhesive flowable resin composites to enamel and dentin were evaluated using a tensile bond test with thermal cycling. Also, the quantities of various ions released from the materials were measured using ICP atomic emission spectrometry and a fluoride ion electrode. The initial bond strengths of the materials were approximately 6 MPa, and decreased after thermal cycling. The S-PRG filler-containing self-adhesive flowable resin composites materials exhibited much higher ion release compared with the commercial self-adhesive flowable resin composite possibly due to different acidic monomers contained. It was suggested that the S-PRG filler containing self-adhesive flowable resin composites should be limited as a lining material or to restore small cavities in non-stress bearing areas.