Formation of filamentous appendages by Acinetobacter sp. Tol 5 for adhering to solid surfaces

Ishii, Shun’ichi; Miyata, Shunsuke; Hotta, Yasuaki; Yamamoto, Kōji; Unno, Hajime; Hori, Katsutoshi

doi:10.1263/jbb.105.20

Cited by 21 publications

(11 citation statements)

References 28 publications

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“…This mutant exhibits decreased adhesion with decreasing ionic strength and does not adhere at all at 0.015 mM, whereas adhesion of the wild type of Tol 5 is fully independent of ionic strength. Even the wild-type strain exhibits ionic strength-dependent adhesion when the cells are grown under culture condition, in which those nanofibers are scarcely produced [64]. These observations imply that adhesion of Tol 5 cells deviates from the DLVO theory due to these nanofibers.…”

Section: Complexity Of Actual Bacterial Adhesionmentioning

confidence: 93%

Bacterial adhesion: From mechanism to control

Hori

Matsumoto

2010

Biochemical Engineering Journal

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Section: Complexity Of Actual Bacterial Adhesionmentioning

confidence: 93%

Bacterial adhesion: From mechanism to control

Hori

Matsumoto

2010

Biochemical Engineering Journal

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640

439

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“…Interestingly, the toluene‐degrading bacterium Acinetobacter sp. Tol 5 generated fimbriae only in the presence of polyurethane foam and toluene as a carbon source (Ishii et al. , 2008).…”

Section: Discussionmentioning

confidence: 99%

Trade‐off between antibiotic resistance and biological fitness in Acinetobacter sp. strain DR1

Kang

Park²

2010

Environmental Microbiology

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Rifampicin, a bactericidal antibiotic drug, is routinely used to make an environmental recipient selective in laboratory-conjugation experiments. We noticed, inadvertently, that the rifampicin-resistant Acinetobacter sp. strain DR1, a recently discovered hexadecane-degrading environmental isolate, exhibited a substantial loss of quorum sensing signalling. The domesticated ampicillin-resistant strain, DR1, evidenced more dramatic phenotypic changes than were observed in the rifampicin-resistant cells: a complete loss of quorum sensing, a loss in swimming and swarming motilities, poor fimbrial expression, increased rigidity in membrane fatty acid composition and reduced hexadecane degradation capability. Interestingly, the motility of strain DR1 grown adjacent to a streptomycin-producing Streptomyces griceus was permanently abrogated, where this change was heritable and other phenotypic changes could not be detected. In this study, we have reported for the first time that the in situ acquisition of antibiotic resistance may reduce biological fitness, including losses in the production of quorum sensing signals, motility and substrate utilization, and each antibiotic is associated with different degrees of phenotypic and genetic alterations. Our data also suggested that the domestication of environmental isolates should be approached with caution, as there are phenotypic variations in antibiotic-resistant cells that might not be noticeable unless all possible phenotypic assays are conducted.

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“…ST‐550 (Doukyu et al, ) was kindly provided by Dr. Noriyuki Doukyu of Toyo University. ST‐550 and its transformants were grown in basal salt (BS) medium (Ishii et al, ) supplemented with 0.03% (v/v) phenol as the sole carbon source or in Luria‐Bertani (LB) medium at 30°C. Antibiotics were used at the following concentrations when required: ampicillin (100 µg mL −1 ) and gentamicin (10 µg mL −1 ) for ST‐550 derivative mutants.…”

Section: Methodsmentioning

confidence: 99%

Application of the adhesive bacterionanofiber AtaA to a novel microbial immobilization method for the production of indigo as a model chemical

Ishikawa

Shigemori

Hori³

2013

Biotech & Bioengineering

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The toluene-degrading bacterium Acinetobacter sp. Tol 5 shows high adhesiveness mediated by the bacterionanofiber protein AtaA, which is a new member of the trimeric autotransporter adhesin (TAA) family. In contrast to other reported TAAs, AtaA mediates the adhesion of Tol 5 to various abiotic surfaces ranging from hydrophobic plastics to hydrophilic glass and stainless steel. The expression of ataA in industrially relevant bacteria improves their adhesiveness and enables immobilization directly onto support materials. This represents a new method that can be alternated with conventional immobilization via gel entrapment and chemical bonding. In this study, we demonstrate the feasibility of this immobilizing method by utilizing AtaA. As a model case for this method, the indigo producer Acinetobacter sp. ST-550 was transformed with ataA and immobilized on a polyurethane support. The immobilized ST-550 cells were transferred directly to a reaction solution containing indole as the substrate. The immobilized ST-550 cells showed a faster indigo production rate at high concentrations of indole compared with planktonic ST-550 not expressing the ataA gene, implying that immobilization enhanced the tolerance of ST-550 to the substrate indole. As a result, the immobilized ST-550 produced fivefold higher levels of indigo than planktonic ST-550. These results proved that AtaA is useful for bacterial immobilization.

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Formation of filamentous appendages by Acinetobacter sp. Tol 5 for adhering to solid surfaces

Cited by 21 publications

References 28 publications

Bacterial adhesion: From mechanism to control

Bacterial adhesion: From mechanism to control

Trade‐off between antibiotic resistance and biological fitness in Acinetobacter sp. strain DR1

Application of the adhesive bacterionanofiber AtaA to a novel microbial immobilization method for the production of indigo as a model chemical

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