BackgroundEsophageal squamous cell carcinoma (ESCC) is a common Malignant tumor of digestive tract which have a potential association with lysosomal pathway. The purpose of this study was to explore the correlation between lysosome pathway and immune infiltration of ESCC.MethodsThe cell type annotation of ESCC patients and the distribution of their gene markers were analyzed by single cell data. They were also grouped according to the expression of lysosomal pathways. Gene set variation analysis (GSVA) enriched pathway scoring, Cellchat cell communication was performed to demonstrate the tumour-associated pathway scores and interactions of different cell populations. Relevant differential genes were screened, prognostic risk markers were constructed and direct associations of lysosomal pathway-related gene risk scores with immune infiltration and tumour treatment drug sensitivity were assessed by algorithms. In cellular experiments, qPCR and flow cytometry were used to assess the role of the lysosomal pathway gene-MT1X on tumour cell development.ResultsESCC single cell data were annotated into 7 Cluster clusters by t-sne downscaling analysis. Cellchat analysis revealed that the “MIF” cellular communication network is the main communication mode of the lysosomal pathway in ESCC cells. The lysosomal pathway genetic risk model was found to be significantly different from ESCC prognosis in both the training and validation groups. The lysosome pathway gene risk model was associated with treatment resistance in ESCC patients using oncopredict R package. The correlation between the expression of lysosomal-DEG and tumour immune infiltration and immune cell types by the MCPcounter method. Cellular assays showed that the lysosomal pathway gene MT1X was less expressed in oesophageal cancer cells than in normal oesophageal epithelial cells. Knockdown of MT1X significantly promoted the growth rate of oesophageal cancer cells.ConclusionBased on the single cell sequencing technology and transcriptomic analysis, we confirmed that there is a close association between the lysosomal pathway and the immune infiltration and treatment sensitivity of ESCC, which may be a potential target for a new direction of ESCC therapy.

No abstract

Ce was successfully doped into the A site of La0.9Mn0.8Ni0.2O3 (LMN) with 5mol% doping content (LCMN-05), and the obtained LCMN-05 acted as catalyst for dry reforming. After reduction in 5%...

No abstract

The paper is divided into three different parts, which use the residue theorem to solve several different integrals, namely, the Euler integral, the Gaussian integral, the Fresnel integral, and so forth. The process of using the resiude theorem to determine these integrals is to first turn the integrals into convenient forms of complex integrals, and then find integral perimeters so that any integral on one of the curves is the required integral, through the drawing observation of the contour to write the original integral into the form of multiple integral. By studying the resiude theorem to solve the problem of complex integrals, it is demonstrated that the resiude theorem is actually a process that makes the calculation easier. These solved integrals have a wide range of applications including the study of the refraction of light, analytics, probability theory, combinatorial mathematics, and unification of the continuous Fourier transform.