Background: Alcohol induced health problems have tormented the world population since time immemorial. This research work was done to study the therapeutic role of Tylophora indica ethanolic extract on alcohol induced anxiety in Wistar albino rats. Method: Wistar albino rats were administered 20% alcohol, orally twice a day for 30 days. The protective role of Tylophora indica ethanolic extract was evaluated by administering it at a dose of 100 mg/kg body weight orally in alcohol treated animals for 30 days. Anxiety was assessed by elevated plus maze and light dark arena tests. After that, animals were sacrificed and brains were dissected out, to measure the levels of dopamine. To pinpoint the active constituent responsible for the anxiolytic activity, Tylophora indica ethanolic extract was subjected to HPLC-LCMS analysis. Results: Elevated plus maze and light dark arena tests showed that alcohol induced anxiety can be prevented by Tylophora indica ethanolic extract in Wistar albino rats. There was a significant increase in the levels of Dopamine in the brains of rats, which received Tylophora indica. From the HPLC-LCMS analysis report it is postulated that Tylophorinidine is the active constituent responsible for the anxiolytic activity of Tylophora indica ethanolic extract. Conclusion: Tylophora indica can mitigate the deleterious effect of alcohol in CNS.

Background:To discover a new agent which possesses dual property of analgesic and antimicrobial activity, thereby reducing the burden of polypharmacy. Phyllanthus amarus was screened for its analgesic and antimicrobial activities.Objectives:The objective was to evaluate the analgesic and antimicrobial activity, of P. amarus ethanolic extract (PAEE).Materials and Methods:The ethanolic extract of P. amarus was prepared using Soxhlet apparatus. An in vivo study using Swiss albino mice was done to screen the central and peripheral analgesic activity of P. amarus extract. The extract was administered at a dose of 100 mg/kg body weight orally. The peripheral analgesic activity was assessed using acetic acid induced writhing test. The central analgesic activity was assessed using Eddy's hot plate apparatus. An in vitro study was carried out to study the antimicrobial activity of the above extract using selected species of Streptococcus mutans, and S. salivarius. The antimicrobial activities were determined using the agar well method.Results:The ethanolic extract of P. amarus showed significant (P < 0.05) peripheral and central analgesic activity. In vitro antimicrobial screening indicated that the ethanolic extract had shown a zone of inhibition against S. mutans and S. salivarius in the agar wells.Conclusion:This study showed that PAEE exhibited significant analgesic and antimicrobial activities.

Background: To find out a new agent with a high therapeutic index for the treatment of anxiety, an indigenous medicinal plant Eucalyptus terteticornis was screened for its effect on anxiety in experimental animal model.Methods: Thirty six adult Wistar albino rats of both sexes weighing 175-200g were divided into three groups: Group I: DMSO 10% (0.1ml/200g), Group II: hexane extract of leaves of Eucalyptus terteticornis (ETHE) (100mg/kg/body weight), Group III: Diazepam (1mg/kg orally). All test compounds were administered orally for ten days. On tenth day, after one hour of test compounds administration, Wistar rats were taken for elevated plus maze (EPM) and light dark arena (LDA) tests. Statistical comparisons among the groups were performed by One-way analysis of variance (ANOVA) followed by Tukey Krammer test.Results: The results showed that ETHE treated animals (Group II) significantly (p <0.001) increased the time spent in open arms of EPM and in bright arena of LDA on comparing with normal (Group I).Conclusions: The anti-anxiety activity of Eucalyptus terteticornis can be due to its effect on brain neurotransmitters or due to antioxidant property.

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