In order to understand the mechanism for maintaining life of animals based on the search of dynamics of bio-molecules, I have developed several sensitive and selective methods for their quantiˆcation. Using the methods of derivatization with the developed benzofurazan ‰uorogenic reagents (4-‰uoro-7-nitro-2,1,3-benzoxadiazole (NBD F), ammonium 7-‰uoro-2,1,3-benzoxadiazole 4-sulfonate (SBD F) and etc.) followed by high-performance liquid chromatography (HPLC)-‰uorescence detection, a certain kind of biological and clinical importances was demonstrated of chiral bio-molecules (D-amino acids, D-lactic acid and so on), peptides and proteins. The proposed method (derivati-zation with SBD F, isolation of the ‰uorescent proteins by two-dimensional HPLC, enzymatic digestion and identiˆca-tion of the altered proteins by HPLC mass spectrometry (MS)/MS with database-searching algorithm) for proteomics studies revieled the changed proteins in the islets of Langerhans of the dexamethazone-induced diabetic rats. An importance of catecholamine metabolism on the blood pressure regulation was also suggested by the method of HPLC chemiluminescence detection of catecholamines and their 3-O-methylmetabolites. A newˆeldnewˆeld of Analytical Chemistry, i.e., Bio-Analytical Chemistry, was also proposed.

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