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Introduction:Due to the influences of family instability in creation and development of behavioral and emotional disorder in children, researchers have urged to study stress as a significant factor in this field. Aim:The present study was Validation of the Parenting Stress Index (PSI) among mothers of preschool children in Tehran. Method:The study was a descriptive and correlation. The statistical population was the parents of children aged 4-6 years old in Tehran and 378 of them were selected. To determine the validity of this index, the CBCL questionnaire and the Blum Quist Growth Scale used. Software was used to analyze the data SPSS Result: The reliability coefficient for Stress Index was 89% and correlation coefficient between parenting stress scale with CBCL questionnaire and Bloom quist's questionnaires was 23%, 68%, respectively. All Correlation coefficients were significant at the 0.01 level Conclusion: Parenting stress index could be used as a reliable tool for the age of 4-6 in clinical and non-clinical studies.