The most frequently used method to quantify microplastics (MPs) visually by microscope is time consuming and labour intensive, where the method is also hindered by the size limitation at 10 µm or even higher. A method is proposed to perform pre-concentration of MPs by vacuum filtration, hydrogen peroxide wet digestion, fluorescent staining and flow cytometric determination to rapidly detect and quantify small MPs sized from 1–50 µm. The method performance was evaluated by the spiking of seven different types of polymer, including polystyrene (PS), low-density polyethylene (LDPE), polypropylene (PP), poly(methyl methacrylate) (PMMA), polyvinyl chloride (PVC), polylactic acid (PLA) and acrylonitrile butadiene styrene (ABS) at different levels (400, 4000, 40,000 particles mL−1), with a satisfactory overall % recoveries (101 ± 19.4%) observed, where in general no significant difference between the two methods was observed. Furthermore, a pre-concentration process by vacuum filtration was introduced to reduce the matrix effect. After pre-concentration, satisfactory % recoveries and accuracy in MP counts resulted from both ultrapure water (94.33 ± 11.16%) and sea water (103.17 ± 9.50%) samples. The validated method using flow cytometry can be used to quantify MPs in environmental water samples that can reduce time and human resources.

The use of medication is effective in managing metabolic syndrome (MetS), but side effects have led to increased attention on using nutraceuticals and supplements. Astaxanthin shows positive effects in reducing the risk of MetS, but results from individual studies are inconclusive. This systematic review summarizes the latest evidence of astaxanthin in adults with risk factors of MetS. A systematic search of English and Chinese randomized controlled trials in 14 electronic databases from inception to 30 June 2021 was performed. Two reviewers independently screened the titles and abstracts, and conducted full-text review, quality appraisal, and extraction of data. Risk of bias was assessed by PEDro. A total of 7 studies met the inclusion criteria with 321 participants. Six studies were rated to have excellent methodological quality, while the remaining one was rated at good. Results show marginal effects of astaxanthin on reduction in total cholesterol and systolic blood pressure, and a significant attenuating effect on low-density lipoprotein cholesterol. Further robust evidence is needed to examine the effects of astaxanthin in adults at risk of MetS.

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Human exposure to microplastics (MPs) through drinking water has drawn serious concern recently because of the potential adverse health effects. Although there are reports on the occurrence of MPs in bottled water, little is known about the abundance of a whole spectrum of MPs with sizes ranging from 1 µm to 5 mm due to the restrictions of conventional MPs detection methods. Some studies using micro-Raman spectroscopy can achieve MPs with a size of <10 µm, however, quantitation of all MPs was extremely time consuming and only a small portion (<10%) of MPs would be analyzed. The present study quantified MPs from nine brands of bottled water using fluorescence microscopy and flow cytometry for MPs with a size of ≥50 µm and a size of <50 µm, respectively. The average abundance of MPs with a size of ≥50 µm in bottled water samples was found ranging from 8–50 particles L−1, while MPs with a size of <50 µm were found to be 1570–17,817 particles L−1, where the MPs abundance from mineral water samples were significantly more than distilled and spring water samples. The modal size and shape of MPs were found at 1 µm and fragments, respectively. Besides, three tap water samples obtained locally were analyzed and compared with the bottled water samples, where less MPs were found in tap water samples. In addition, contamination of MPs from bottle and cap and interference by addition of mineral salts were studied, where no significant difference from all these processes to the control sample was found, suggesting the major contamination of MPs was from other manufacturing processes. Estimated daily intake (EDI) of MPs increased substantially when data of small MPs are included, suggesting that previously reports on exposure of MPs from drinking water might be underestimated, as only large MPs were considered.

The use of probiotic bacteria can not only enhance the nutritional utilization of fish feeds to produce more biomass but can also provide a practically “safer” alternative to the fish farming industry to reduce the abuse of antibiotics and drugs. This study investigated the possibility of colonizing Lactobacillus rhamnosus strain GG (LGG) to the intestine of Cirrhinus molitorella (mud carp) fingerling. Colonization of LGG was observed in gut tissue after 14 days of administration with a diet supplemented with 1 × 108 CFU/mL LGG. Moreover, growth performance parameters of the LGG-supplemented diet group, including relative weight gain, feed conversion ratio and feed efficiency, were found about two-fold higher than the control group after 60 days. In addition, fish fed with an LGG-supplemented diet for 60 days showed substantial resistance against the infection of pathogenic bacterial Aeromonas hydrophila, with a relative survival rate of up to 57% compared to the control group. In summary, the results indicated that LGG as dietary supplement for mud carp fingerling can enhance nutrition utilization and better protect fish against the infection of Aeromonas hydrophila. The results provide an insight to the fish farming industry, encouraging a reduction in the use of antibiotics and drugs and the production of “safer” mud carp for the market at a manageable cost.