Pseudorabies, caused by pseudorabies virus (PRV), is the main highly infectious disease that severely affects the pig industry globally. T-2 toxin (T2), a significant mycotoxin, is widely spread in food and feeds and shows high toxicity to mammals. The potential mechanism of the interaction between viruses and toxins is of great research value because revealing this mechanism may provide new ideas for their joint prevention and control. In this study, we investigated the effect of T2 on PRV replication and the mechanism of action. The results showed that at a low dose (10 nM), T2 had no significant effect on porcine kidney 15 (PK15) cell viability. However, this T2 concentration alleviated PRV-induced cell injury and increased cell survival time. Additionally, the number of PK15 cells infected with PRV significantly reduced by T2 treatment. Similarly, T2 significantly decreased the copy number of PRV. Investigation of the mechanism revealed that 10 nM T2 significantly inhibits PRV replication and leads to downregulation of oxidative stress- and apoptosis-related genes. These results suggest that oxidative stress and apoptosis are involved in the inhibition of PRV replication in PK15 cells by low-concentration T2. Taken together, we demonstrated the protective effects of T2 against PRV infection. A low T2 concentration inhibited the replication of PRV in PK15 cells, and this process was accompanied by downregulation of the oxidative stress and apoptosis signaling pathways. Our findings partly explain the interaction mechanism between T2 and PRV, relating to oxidative stress and apoptosis, though further research is required.

Existing electronic devices will quickly become e-waste when encountering technological iterations, which results in serious environmental and public health problems. Previous circular economy research has mainly focused on the development of new products with long life or recycling discarded products. This study firstly proposes the Green-Extension Design (GED) strategy for developing adaptable accessories that provide existing products with the ability to continue to work in a different context. Competitiveness was selected to evaluate the performance of GED, and three competitiveness components were derived through principal component analysis (PCA). Moreover, AHP (Analytic Hierarchy Process) was applied to define the weights of the three competitiveness components, and a GED model was established on the basis of production function. Furthermore, the calculation method for each competitiveness component was defined. The GED strategy is aimed at extending the life of existing products, as well as reducing resource waste and environmental pollution. The GED model based on competitiveness components can enable enterprises to design products of high competitiveness and obtain market share as a result.

To achieve energy and environmental policy goals, profound social changes have taken place in the Chinese society. Existing relevant research on energy assessment tends to focus on their respective fields, and policy-level support often requires an assessment of predictable effects based on the overall perspective. In response to this problem, this paper carries out an interdisciplinary study. We chose green design as a new perspective to assess this process. Green design has made important contributions to the sustainable development in the fields of building, construction, and urban planning, which deeply affect the energy consumption in the world. By combing through the development concepts and models of green design in various fields, a comprehensive index—green design index (hereafter referred as to GDI)—is first introduced. Further, a multi-level big data structure of GDI has been built and the development of green design in regions of China based on long-term series is quantitatively assessed. The results of this study show that green design in regions of China has been greatly improved during the inspection period, but there are huge regional differences. The required policies and implementation routes also need to be adapted to local conditions. This paper first introduces a credible quantitative analysis framework of green design, and a corresponding research foundation for energy transition research based on green design.

Pseudorabies, caused by pseudorabies virus (PRV), is the main highly infectious disease that severely affects the pig industry globally. T-2 toxin (T2), a significant mycotoxin, is widely spread in food and feeds and shows high toxicity to mammals. The potential mechanism of the interaction between viruses and toxins is of great research value because revealing this mechanism may provide new ideas for their joint prevention and control. In this study, we investigated the effect of T2 on PRV replication and the mechanism of action. The results showed that at a low dose (10 nM) T2 had no significant effect on porcine kidney 15 (PK15) cell viability. However, this T2 concentration alleviated PRV-induced cell injury and increased cell survival time. Additionally, the number of PK15 cells infected with PRV was significantly reduced by T2 treatment. Similarly, T2 significantly decreased the copy number of PRV. Investigation of the mechanism revealed that 10 nM T2 significantly inhibited PRV replication and led to downregulation of oxidative stress- and apoptosis-related genes. These results suggest that oxidative stress and apoptosis are involved in the inhibition of PRV replication in PK15 cells by low-concentration T2. Taken together, we demonstrated the protective effects of T2 against PRV infection. A low T2 concentration inhibited the replication of PRV in PK15 cells, and this process was accompanied by downregulation of by the oxidative stress and apoptosis signaling pathways. Our findings partly explain the interaction mechanism between T2 and PRV, relating to oxidative stress and apoptosis, though further research is required.