Bruxelas' e 'Tronchuda Portuguesa', as pimentas 'Dedo de Moça', 'Malagueta', 'Doce Italiana', 'Jalapeño M', 'Amarela Comprida' e 'Cambuci', os pimentões 'Dagmar', 'Casca Dura Ikeda', 'Magna Super' e os porta enxertos para pimentão 'Silver' e 'AF 8253'. As plantas resistentes a M. javanica foram: alface 'Roxa', cebolinhas 'Tokyo' e 'Nebuka', alho 'Poró Gigante', salsa 'Lisa Comum', brócolis 'Brasília', azevém, C. spectabilis, C. juncea, C. breviflora, girassol 'Uruguai', guandu anão, milheto e mucuna preta. Palavras-chave: nematoides das galhas, olerícolas, resistência, suscetibilidade. ABSTRACT Meloidogyne javanica reproduction on vegetable crops and plants used as green manureThe goal of this work was to determinate the Meloidogyne javanica reproduction factor on vegetable crops (Allium porrum, A. schoenoprasum, Beta vulgaris, Brassica spp., Capsicum spp., Coriandrum sativum, Daucus carota, Lactuca sativa, Petroselinum crispum and Raphanus sativus) and on plants used as green manure (Cajanus cajan, Crotalaria breviflora, C. juncea, C. mucronata, C. ochroleuca, C. spectabilis, Dolichos lalab, Helianthus annuus, Lollium multiflorum, M. aterrima, M. cinereum, Mucuna deeringiana, Pennisetum glaucum and Raphanus sativus). The experiments were carried out in a greenhouse during 60 days. The substrate infestation was made with 5,000 eggs and possible second stage juveniles of M. javanica. The immune plants to M. javanica were: P. crispum 'Portuguesa', Brassica spp.

Entomopathogenic nematodes (EPNs) (Steinernematidae and Heterorhabditidae) can control pests due to the mutualistic association with bacteria that kill the host by septicemia and make the environment favorable for EPNs development and reproduction. The diversity of EPNs in Brazilian soils requires further study. The identification of EPNs, adapted to environmental and climatic conditions of cultivated areas is important for sustainable pest suppression in integrated management programs in agricultural areas of Brazil. The objective was to identify EPNs isolated from agricultural soils with annual, fruit and forest crops in Brazil. Soil samples were collected and stored in 250 ml glass vials. The nematodes were isolated from these samples with live bait traps ([Galleria mellonella L. (Lepidoptera: Pyralidae) larvae]. Infective juveniles were collected with White traps and identified by DNA barcoding procedures by sequencing the D2/D3 expansion of the 28S rDNA region by PCR. EPNs identified in agricultural areas in Brazil were Heterorhabditis amazonensis, Metarhabditis rainai, Oscheios tipulae and Steinernema rarum. These species should be considered pest biocontrol agents in Brazilian agricultural areas.

The objective of this research was to verify the resistance response of eight Brazilian commercial tomato rootstocks to the nematode Meloidogyne mayaguensis. The studied rootstocks were: ' G u a r d i ã o ' , ' H e l p e r-M ' , ' A n c h o r-T ' , ' D r. K ' , ' K a g e m u s c h a ' , 'TMA 809', 'Magnet' and 'He-Man'. The experiment consisted of 9 treatments (8 rootstocks and 'Rutgers' used as susceptible control), with 6 replication. Each plot was constituted by 1 plant Cantu, R.R.; Wilcken, S.R.S.; Rosa, J.M.O. & Goto, R.. Reaction of commercial tomato rootstocks plant to Meloidogyne mayaguensis. Summa Phytopathologica, v.35, n.3, p.216-218, 2009 Keywords: tomato plant, grafting, root-knot nematode. per pot, maintained in green-house. The plants were inoculated with 5,000 eggs and second stage juvenile of M. mayaguensis. T h e e x p e r i m e n t a l d e s i g n w a s c o m p l e t e l y r a n d o m i z e d . T h e evaluation was 60 days after inoculation, when the root weight, gall and egg mass index, nematode number in soil and in root and reproduction factor were evaluated. All studied rootstocks were susceptible to M. mayaguensis. PalavrasAs doenças de solo possuem grande importância na cultura do tomateiro, pelos danos causados e pelas dificuldades no controle. Dentre estas destacam-se os nematóides parasitos de plantas, que em muitos casos inviabilizam a produção e o cultivo em áreas infestadas. As plantas de tomateiro quando atacadas severamente pelo nematóide das galhas, Meloidogyne spp., apresentam o sistema radicular completamente desorganizado e com poucas raízes funcionais. Em altas infestações do nematóide no início da cultura pode ocorrer a morte de mudas no campo, e nas plantas sobreviventes a produção é fortemente afetada em quantidade e qualidade (1, 9).Algumas técnicas vêm sendo recomendadas no controle dos nematóides parasito de plantas, a exemplo da utilização de adubos verdes em sistemas de rotação de culturas, da utilização de organismos supressores aos nematóides, assim como a utilização da solarização e, quando possível, o uso de cultivares resistentes (2,11,16).No controle de patógenos do solo, a utilização da enxertia tem se mostrado interessante, pois não exige uma mudança drástica no manejo da cultura (14). Por esse motivo, o uso da enxertia em tomateiros, com o emprego de porta-enxertos resistentes a nematóides do gênero Meloidogyne, tem sido recomendado em áreas infestadas por esses patógenos (18). Entretanto, essa técnica, quando utilizada visando principalmente o controle de patógenos do solo, pode ser inviabilizada em razão dos problemas ocasionados pelo surgimento de outras espécies ou até mesmo de raças, estirpes e biovares dentro de uma dada espécie de patógeno (13).A espécie Meloidogyne mayaguensis foi descrita a partir de população encontrada em raízes de Solanum melongena oriundas da região noroeste de Porto Rico, inicialmente identificada como Meloidogyne arenaria. Neste estudo, os nematóides apresentaram características morfológicas, citogenéticas e bioquím...

Reprodução de Meloidogyne enterolobii em olerícolas e plantas utilizadas na adubação verde 1Meloidogyne enterolobii reproduction on vegetable crops and plants used as green manure

PRESSURIZATION OF THE SPRAYING SUSPENSION AND VIABILITY OF ENTOMOPATHOGENSABSTRACT: The objective of this research was to evaluate the influence of spraying pressurization upon the viability of the following entomopathogens microorganisms: the fungus Metarhizium anisopliae, the bacterium Bacillus thuringiensis var. kurstaki, the nucleopolyhedrovirus of Anticarsia gemmatalis (AgMNPV), and the nematode Steinernema glaseri. The sprayer hydraulical system was used without filters and equipped with JA-2 hollow-cone nozzle type. The experimental design was completely randomized, with seven treatments: pressurization of spraying suspension at 172; 345; 517; 689; 1,034 and 1,379 MPa, and check (suspension not submitted to the pressurization). Evaluation of the viability of M. anisopliae was estimated by the percentage of germinated conidia. For B. thuringiensis, by the number of colonies formed. Efficiency on AgMNPV was estimated indirectly, by means of mortality caused to A. gemmatalis infected as early third-instars larvae reared in an artificial diet impregnated with the viral suspension. For the experiment with S. glaseri it was calculated the percentage of live specimens on Peters slide-blade. It concluded that the entomopathogens M. anisopliae, B. thuringiensis, AgMNPV and S. glaseri can be sprayed up to the pressure of 1,379 MPa without losing the viability of those organisms as biological control agents.