Highlightsd An ultrafast deconvolution tool for top-down mass spectrometry data is presented d A spectrum transformation that dramatically accelerates deconvolution is suggested d Our method reports more masses and substantially fewer artifacts than other tools

Abstrak -Telah dilakukan penelitian dan pengembangan media pembelajaran laboratorium virtual yang bertujuan untuk mengatasi miskonsepsi peserta didik materi fisika inti dan radioaktivitas Subjek uji coba adalah peserta didik kelas XII IPA SMA Binamu berjumlah 30 orang. Prosedur pengembangannya menggunakan model Four-D terdiri dari tahap pendefinisian, perancangan, dan pengembangan. Hasil penelitian menunjukkan (1) media laboratorium virtual model presentasi dan tutorial pada materi aktivitas zat radioaktif dan daya tembus sinar radioaktif diperoleh hasil valid dan reliabel. Setiap tampilan dilengkapi navigasi, petunjuk, hyperlink,dan fasilitas lainnya untuk memudahkan penggunaan program, (2) perangkat pembelajaran berupa Rencana Pelaksanaan Pembelajaran, Buku Bacaan, dan Lembar Kerja Peserta Didik selain dibuat dalam bentuk hardcopy, juga softcopy autorun CD. Penilaian menunjukkan valid dan reliabel, (3) aktivitas peserta didik di atas 85%, menunjukkan pembelajaran yang dilakukan mampu mengaktifkan peserta didik. Persentase persepsi peserta didik adalah 93,05% menunjukkan sangat setuju terhadap pembelajaran fisika berbasis media laboratorium virtual, (4) berdasarkan tes akhir, terjadi peningkatan pemahaman konsep yang baik peserta didik dibandingkan sebelum diberi media laboratorium virtual. Abstract -The develovment research of learning instrument of virtual laboratory was conducted which aimed at contending themisconception about nuclear physics. The subject of the study was 30 students grade XII IPA at SMA 1 Binamu,Jeneponto.The procedure of development referred to four-D model consisted of definition phase, design phase, and development phase. The result of the study revealed that (1) the virtual laboratory media developed in form of presentation and tutorial model that consisted of radioactivity-decay and emerge power of radioactive radiation gotten valid and reliable result.each of media display completed with navigation, program instruction, hyperlink and several other facilities to allow users to run the program.(2)the learning instrument in form of lesson plan, the textbook, and student's workbook presented in softcopy of CD autorun.product was valid and reliable. (3) the student's activities during the learning process were above 85%, show that the students agree with instrument physiscs learning basis on virtual laboratory. (4) according to the tes result, the concept comprehension of students was improve than before given virtual laboratory media.

Feature deconvolution, the determination of intact proteoform masses, is crucial for top-down proteomics, but currently suffers from long runtimes and quality issues. We present FLASHDeconv, an algorithm based on a simple transformation of mass spectra, which turns deconvolution into the search for constant patterns thus greatly accelerating the process. We show higher deconvolution quality and two to three orders of magnitude faster execution speed than existing approaches.

The demand for reliable comparability studies of biosimilars grows with their increased market share. These studies focus on physicochemical, structural, functional and clinical properties to ensure that a biosimilar has no significant differences to the originator product and can be released into the market without extensive clinical trials. In the current study, Enbrel® (etanercept, the originator) and Altebrel™ (the proposed biosimilar) underwent direct comparison. “Bottom-up” mass spectrometric analysis was used for primary sequence analysis, evaluation of N/O-glycosylation sites and quantification of methionine oxidation. N/O-glycans were analyzed after permethylation derivatization and the effect of N-glycans on in-vitro functionality of etanercept was assayed. Three enzyme peptide mapping resulted in complete identification of the primary structure. It was confirmed that total ion chromatograms are valuable datasets for the analysis of the primary structure of biodrugs. New N/O-glycan structures were identified and all the N-glycans were quantified. Finally, investigation of the functional properties of N-deglycosylated and non-modified etanercept samples using surface plasmon resonance analysis and in-vitro bioassay showed that N-glycosylation has no significant effect on its in-vitro functionality. Analysis of etanercept and its biosimilar, revealed a high similarity in terms of glycosylation, primary structure and in-vitro functionality.