Systematically organizing the anatomical, molecular, and physiological properties of cortical neurons is important for understanding their computational functions. Hippocampome.org defines 122 neuron types in the rodent hippocampal formation based on their somatic, axonal, and dendritic locations, putative excitatory/inhibitory outputs, molecular marker expression, and biophysical properties. We augmented the electrophysiological data of this knowledge base by collecting, quantifying, and analyzing the firing responses to depolarizing current injections for every hippocampal neuron type from published experiments. We designed and implemented objective protocols to classify firing patterns based on 5 transients (delay, adapting spiking, rapidly adapting spiking, transient stuttering, and transient slow-wave bursting) and 4 steady states (non-adapting spiking, persistent stuttering, persistent slow-wave bursting, and silence). This automated approach revealed 9 unique (plus one spurious) families of firing pattern phenotypes while distinguishing potential new neuronal subtypes. Novel statistical associations emerged between firing responses and other electrophysiological properties, morphological features, and molecular marker expression. The firing pattern parameters, experimental conditions, spike times, references to the original empirical evidences, and analysis scripts are released open-source through Hippocampome.org for all neuron types, greatly enhancing the existing search and browse capabilities. This information, collated online in human- and machine-accessible form, will help design and interpret both experiments and model simulations.

The diversity of intrinsic dynamics observed in neurons may enhance the computations implemented in the circuit by enriching network-level emergent properties such as synchronization and phase locking. Large-scale spiking network models of entire brain regions offer a platform to test theories of neural computation and cognitive function, providing useful insights on information processing in the nervous system. However, a systematic in-depth investigation requires network simulations to capture the biological intrinsic diversity of individual neurons at a sufficient level of accuracy. The computationally efficient Izhikevich model can reproduce a wide range of neuronal behaviors qualitatively. Previous studies using optimization techniques, however, were less successful in quantitatively matching experimentally recorded voltage traces. In this article, we present an automated pipeline based on evolutionary algorithms to quantitatively reproduce features of various classes of neuronal spike patterns using the Izhikevich model. Employing experimental data from Hippocampome.org, a comprehensive knowledgebase of neuron types in the rodent hippocampus, we demonstrate that our approach reliably fit Izhikevich models to nine distinct classes of experimentally recorded spike patterns, including delayed spiking, spiking with adaptation, stuttering, and bursting. Importantly, by leveraging the parameter-exploration capabilities of evolutionary algorithms, and by representing qualitative spike pattern class definitions in the error landscape, our approach creates several suitable models for each neuron type, exhibiting appropriate feature variabilities among neurons. Moreover, we demonstrate the flexibility of our methodology by creating multi-compartment Izhikevich models for each neuron type in addition to single-point versions. Although the results presented here focus on hippocampal neuron types, the same strategy is broadly applicable to any neural systems.

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