Antibiotic pollution has become one of the most challenging environmental issues in aquatic ecosystems, with adverse effects on planktonic organisms that occupy the base of the aquatic food chain. However, research regarding this topic has not been systematically reviewed, especially in terms of community-level responses. In this review, we provide an overview of current antibiotic pollution in aquatic environments worldwide. Then, we summarize recent studies concerning the responses of planktonic communities to antibiotics, ranging from individual- to community-level responses. Studies have shown that extremely high concentrations of antibiotics can directly harm the growth and survival of plankton; however, such concentrations are rarely found in natural freshwater. It is more likely that environmentally relevant concentrations of antibiotics will affect the physiological, morphological, and behavioral characteristics of planktonic organisms; influence interspecific interactions among plankton species via asymmetrical responses in species traits; and thus alter the structure and function of the entire planktonic ecosystem. This review highlights the importance of community analysis in revealing antibiotic toxicity. We also encourage the establishment of the causal relationships between impacts at multiple scales in the future for predicting the community-level consequences of antibiotics based on the currently available individual-level evidence.