The aim: The purpose of the study is to characterize the influence of quantitative and qualitative composition of gingival microbiota on the status of the main immune system cells, localized in the gums, in chronic generalized catarrhal gingivitis in children. Materials and methods: The study involved 26 children aged 9 to 16 years, patients with chronic generalized catarrhal gingivitis mild to moderate severity (CGCG) and 18 children with intact gums were comparison group. We determined the hygienic indices Fedorov, has been received, Silness-Loe, PMA, bleeding index for Myuleman and intensity of caries index CFD + cf, CFD. Histological and immunohistochemical studies were performed on serial sections kriostatnyh who made biopsy of gingival papillae. Microbiological study gingival part of crown plaque was performed by multiplexed PCR in real time. Results: Value hygienic indices in children with CGCG higher than in healthy, indicating the difficulty of care in the presence of periodontal inflammation. As a result of immunohistochemical studies revealed that HLA-DR + cells under conditions of active disease migrate to mucosal lamina propria epithelium. Number of CD3 + cells in the epithelium CGCG was significantly higher than the number in the intact epithelium and was the most numerous of population. In the biopsy of affected children significantly reduced the number of CD4 + cells. When CGCG quantitative total bacterial mass, Lactobacillus spp., Enterobacteriaceae, Gardnerella vaginalis / Prevotella bivia / Porphyromonas spp. in the sample CROWN dental plaque was significantly higher than rates under physiological conditions, and may serve as diagnostic criteria of dysbiosis. Conclusions: So, CGCG is a disease in the etiology of which is one of the leading roles played by microbial factor, namely, the value of the quantitative ratios of certain types of microorganisms of dental plaque compared to the total bacterial mass of plaque. Therefore, it is reasonable to include comprehensive treatment CGCG drugs in children, leading to natural immunostimulation which causes restoration of local immunity in the gum tissue and drugs to restore quantitative and qualitative composition of normal microflora of the child, thus providing a high therapeutic effect and serve as justification their choice.

The aim: To determine the structure of acute injuries of temporary and permanent frontal teeth in children, to analyze the applied diagnostic and treatment measures for acute tooth trauma and to investigate their effectiveness. Materials and methods: The subject of the study were 31 children aged 2-12 years with acute temporary and permanent tooth trauma. Methods: clinical (radiological, thermodiagnosis), medical and statistical. Results: The number of injured permanent teeth was greater than the number of injured temporary teeth. The pattern of temporary and permanent tooth injuries differed, so in temporary teeth dislocations were observed, and in permanent teeth fractures prevailed over dislocations. In our opinion, this is due to the peculiarities of the anatomy of the temporary teeth. Usually, the therapeutic tactics of acute temporary teeth injuries is to remove them, despite their important role in the growth and development of jaws, physiological formation and eruption of permanent teeth. The therapeutic tactics of dynamic observation in the case of intrusive dislocation of the temporary tooth were selected in the clinic of the Department of Pediatric Dentistry. In some cases, with a slight change in the position of the temporary tooth, self-regulation of its position was observed, in other cases the tooth remained dystopic, but its viability remained in 50% of cases. Therapeutic tactics of acute injuries of permanent teeth were selected according to the type of trauma. In the case of permanent tooth dislocation, with a slight change in its position, tooth immobilization was carried out by splinting with fiberglass tape and photopolymer composite material. Conclusions: Our observations have shown that the complex of modern specialized medical care for the affected children with acute traumatic injuries of the teeth should be guided by their preservation, which ensures the subsequent normal formation of the dental-jaw apparatus. To ensure these conditions, a long, reliable immobilization of the damaged tooth is required as soon as possible after injury.

The aim: To establish the characteristics of the gingival mucosa cellular composition in school-age children. Materials and methods: We made a dental and cytological examination on 150 Ukrainian (Poltava city) children aged 6, 12, and 15 years. Smears were got by scraping from the gum mucosa and stained with a May-Grunwald solution. Cytograms were made by using a Biorex-3 BM-500T microscope with a DCM-900 digital microphotographic attachment. The programs were adapted for these types of studies. We used a magnification of 1000. The t-test for paired samples was used to compare values. The difference was considered statistically significant at P<0.05. Results: The number of superficial epitheliocytes in children aged 12 and 15 was significantly higher than in 6-year-olds but did not differ from each other. Conclusions: The process of physiological keratinization of the cells of the gingival mucosa in schoolchildren is diverse and keratinization occurs both due to the phenomenon of physiological necrosis, namely, apoptosis of surface cells and due to orthokeratosis. From the age of 6, the average number of surface cells in cytograms decreases, while the number of intermediate and dead keratinized cells increases up to 12 years and remains on the previous level in children of 15 years.