Breast cancer is the most common cancer in women throughout the world, with new cases and deaths which continue to increase. Soursop leaves (Annona muricata L) have been used extensively in traditional medicine, including cancer. Acetogenin, alkaloids, and phenols contained in soursop leaves are known to have anti-cancer effects. Among them, acetogenin has the most dominant role and reported to have a cytotoxic effect on various cancer cell lines. This study aims to determine the cytotoxic activity of soursop leaf ethanol extract on T47D breast cancer cell line. Measurement of cytotoxic activity was carried out by the MTT method, and the viability percentage of T47D cells was calculated based on the absorbance values in the treatment, cell control, and media control groups of each replicate. The correlation between extract concentration and viability percentage of the T47D cell line was outlined in the regression equation to obtain the IC50 value. IC50 values of 109.91 ± 3.04 with R values 0.975 and R2 0.9508 obtained. R values close to 1 indicated a strong correlation between extract concentration and the percentage of living T47D cells. Meanwhile, the amount of R2 suggested that the level of AMEE had a 95.08% influence on the rate of cell viability, and the other 4.92% influenced by factors other than the AMEE dose. These results indicated that the ethanol extract of soursop leaves has a cytotoxic effect and has the potential to inhibit T47D cell proliferation in vitro.

Extract is basic material for herbal drug. Herbal drugs formulation requires consistent of biological activity, a consistent chemical profile, or simply a quality assurance programs that can be achieved by standardizing the extracts. The leaves of tahongai (Kleinhovia hospita L.) have been traditionally used in Komering ethnic groups as phytotherapy to cure the inflammation related diseases including cancer, furuncles, polyps, and tonsillitis. The aim of this study was to standardize the quality of ethanolic extract of tahongai leaves by determining the specific and non specific parameters of the extract. The preliminary phytochemical analysis revealed presence of alkaloids, flavonoids, saponins, tanins, and steroids in the extract. The result of specific parameters analysis of the extracts showed that the organoleptic properties of ethanolic extract of tahongai leaves were thick, brownish black, has distinctive odor, astringent with slightly bitter taste, the water and ethanol soluble extractive content were 19.263% ± 0.95 and 18.30% ± 0.51 respectively. The nonspecific parameters analysis of the extract showed that the extract's density was 1.413 g/mL ± 0.04, the water content value was 21.16% ± 0.55, total ash content was 15.64% ± 0.75, acid insoluble ash content was 8.282% ± 0.28, Pb contamination content was 3.67 ppm, Cd contamination content was <0,0043 ppm, total bacteria contamination was 90.5 x 10 1 colony/g, and the total mold and yeast contamination was 1 x 10 1 colony/g.

Perubahan klinis pada payudara sebagian besar bersifat jinak, hanya 3 – 6% yang merupakan keganasan payudara. Namun demikian, kanker payudara menjadi perhatian utama mengingat angka kejadian yang terus menerus meningkat serta morbiditas dan mortalitas yang tinggi. Pengobatan kanker hingga saat ini masih menjadi tantangan. Hal ini membuka peluang penelitian terapi kanker terutama dari bahan herbal. Daun sirsak telah banyak digunakan masyarakat secara tradisional untuk pengobatan tumor. Di samping itu berbagai studi juga telah menunjukkan efek sitotoksik tanaman ini terhadap berbagi cell line kanker. Namun demikian belum ada penelitian yang menganalisis efek ekstrak daun sirsak terhadap populasi limfosit di lingkungan mikro tumor terutama yang berfungsi pada respon imun anti tumor. Penelitian ini bertujuan untuk mengetahui infiltrasi sel limfosit T CD8 pada jaringan tumor yang payudara tikus Sparaque Dawley yang diberikan ekstrak etanol daun sirsak. Ekspresi CD8 dianalisis melalui pewarnaan imunohistokimia. Ekspresi CD8 positif ditandai dengan warna coklat pada sitoplasma sel limfosit, selanjutnya ekspresi CD8 dari setiap sampel dihitung pada 3 lapang pandang dengan high-power field, kemudian ditentukan nilai rata-ratanya. Pewarnaan HE menunjukkan variasi infiltrasi limfosit pada nodul payudara tikus SD. Rata-rata ekspresi CD8 kelompok kontrol menunjukkan hasil lebih tinggi dibandingkan kelompok perlakuan. Namun demikian, hasil uji komparasi menunjukkan tidak ada perbedaan bermakna  (p > 0,05) antara ekspresi CD8 kelompok kontrol dengan perlakuan. Hasil ini menunjukkan bahwa pemberian ekstrak daun sirsak tidak menyebabkan perbedaan bermakna ekspresi CD8 pada populasi limfosit yang menginfiltrasi tumor payudara tikus SD.

Gaya hidup kurang gerak dikombinasikan dengan asupan kalori yang berlebihan menghasilkan penyimpanan energi ekstra sebagai lemak. Prevalensi sindrom metabolik diperkirakan akan berkembang secara substansial dalam skala global. Pasien dengan sindrom metabolik memiliki resiko terkena stroke 3-4x lebih besar. Sebanyak 34% penduduk di Ulak Kerbau Baru memperoleh penghasilan dari pertanian dan perkebunan. Ubi jalar ungu mengandung jauh lebih banyak antosianin daripada ubi jalar kuning dan putih. Antosianin dapat menjaga kesehatan dan menurunkan bahaya penyakit degeneratif, aktivitas antikanker, kapasitas antioksidan, aktivitas antiulcer, dan gangguan kardiovaskular. Pemahaman masyarakat tentang manfaat ubi ungu bagi kesehatan masih terbatas. Program pengabdian masyarakat dengan sistem perkuliahan desa dapat menjadi sarana peningkatan pemahaman masyarakat. Kegiatan pengabdian dilakukan dengan metode penyuluhan dan demo pembuatan produk pangan sehat yang berbahan dasar tepung ubi jalar ungu. Peningkatan persentase jawaban dari pre-test (33,913%) ke post-test (87,273%) menunjukkan bahwa informasi diterima dengan baik oleh masyarakat desa Ulak Kerbau Baru. Hasil penilaian organoleptik masyarakat dimana mayoritas responden menilai suka dan sangat suka pada produk olahan tepung ubi jalar ungu dengan parameter rasa, aroma, tekstur, warna, dan bentuk.   A sedentary lifestyle combined with an excessive caloric intake results in the storage of extra energy as fat. The prevalence of metabolic syndrome is predicted to expand substantially on a global scale. Patients with metabolic syndrome have a 3-4 times increased risk of having a stroke. The majority of residents in Ulak Kerbau Baru derive their income from agriculture and plantations. Purple sweet potatoes contain far more anthocyanins than their orange and white counterparts. Anthocyanins can preserve health and lower the danger of degenerative diseases, anticancer activity, antioxidant capacity, antiulcer activity, and cardiovascular disorders. The public's understanding of the health benefits of purple sweet potato remains limited. Through this community service program and the village lecture plan, it is anticipated that public awareness of metabolic syndrome disease will rise. Using lecture approaches that are integrated with electives courses in Traditional Medicine, knowledge is enhanced. The increase in the percentage of accurate responses from the pre-test (33,913%) to the post-test (87,273%) indicates that the information is well received by the general population. The results of the organoleptic assessment of the community, in which the majority of respondents rated the parameters of taste, scent, texture, colour, and shape of the product as like or liking very much, indicate that people prefer the processed items that are produced.