Bouea macrophylla Griff. is well-known as one of native typical fruits in Southeast Asia which needs to be preserved and continuously cultivated because of economical and ecological significances. More recently, sequence-related amplified polymorphism (SRAP) markers have been developed, which are used to amplify coding regions of DNA with primers targeting open reading frames. This technique has proven to be robust and highly variable and is attained through a significantly less technically demanding process. In this research, SRAP method was preliminary applied to assess genetic characterization of B. macrophylla. Genomic DNA was extracted from fresh leaf samples. The result clearly showed that at 100 ng template DNA and MgCl 2 5 mM concentration are suitable for further PCR analysis. Thirty SRAP primer combinations were initially screened for analysis and 26 primer combinations were chosen for further analysis. A total of 222 DNA fragments, varying from 90-2500 bp, were amplified. The produced band number for each optimal primer set ranged from 3 to 12 with a percentage of polymorphic bands spanning from 33.33 to 80.00%. Therefore, SRAP analysis is suitable for further analysis method on genetic study of Bouea species and related genera.

Abstract. Suyatna I, Sidik AS, Almadi FA, Rizal S, Sukarti K. 2016. Fish community structure in high water temperature around Bontang Industrial Estate, East Kalimantan, Indonesia. Biodiversitas 17: 558-564. We have conducted studies on fish community and their physical and chemical properties of coastal waters in the work area of the industrial estate from 2012 to 2014. At least four industries are known to use the coastal waters in cooling their processing equipments, the hot wastewater is then discharged back into the coastal environment. The current study reports the result of a survey undertaken around the work area of PT Blackbear Resources Indonesia. The result showed that high water temperature and tidal fluctuation are affecting the fish community in the area. By sampling results, during HWL of the spring tide at the maximum of water temperature of 35,9 o C, fish were the most populated and found 5999 ind. or 72,4% of the total number of 8291 fish. During LWL of the same tide type fish were observed 1931 ind (23.3%) and during the neap tide 361 ind (4.3%) at the maximum of water temperature of approximately 40 o C, this ambient showed that the fish population drastically change in number and the environment became undesirable.