Helminth infections are among the most widespread infections in humans. Ficus benghalensis L.

(Moraceae) is traditionally used in Indian system of medicine. Anthelmintic activity was evaluated using worms as experimental models. Test sample of Ficus benghalensis aerial roots extracts was prepared at the concentration 10, 25, 50mg/ml in normal saline solution. Standard drug used Albendazole (20mg/ml). Time for death of worms were recorded after ascertaining that worms neither moved when shaken vigorously nor when dipped in warm water (50^o c). Phytochemical constituents such as Phenolics, tannins, saponins, mucilage and alkaloids may be responsible for the anthelmintic activity. It may be concluded that methanolic extract of Ficus benghalensis possesses significant anthelmintic property.

A fast, simple, reliable and accurate RPHPLC analytical method was developed for the evaluation of botulinum toxin, and the developed method was subsequently validated according to ICH guidelines in sterile dosage form for stability studies. A C18 column with a flow rate of 2 ml/min was selected. The mobile phase chosen consisted of sodium phosphate buffer (0.05 M) at pH 2.8 and acetonitrile in a ratio of 30:70 respectively at 214 nm. Measured at an Rt of 2.1 min. 10 minutes running time. Linearity and range were observed at concentrations from 1 µg/ml to 10 µg/ml. The method developed was linear with a correlation coefficient of 0.99. Validation of the method was performed according to ICH guidelines for assay, linearity and range, precision, limit of detection, limit of quantitation, and forced degradation test.