No abstract

Cancer is a class of diseases that is characterized by the uncontrollable or unstoppable growth of abnormal cells with the potential to invade or spread to other normal body parts. According to WHO estimates, globally 10 million new cancer cases are diagnosed each year. Its 100% prevention becomes a major challenge for the scientific fraternity. Therefore, there is an urgent need to discover new drugs that could overcome the present issue. In order to meet the challenges, a library of 22 novel 1H‐1,2,3‐triazol‐4‐yl‐methyl tethered 3‐pyrrolyl isatin derivatives have been synthesized and well characterized by using different spectral techniques. The newly synthesized conjugates were screened for their anti‐proliferative activity against breast cancer MCF‐7 and MDA‐MB‐231, as well as human embryonic HEK 293 cell lines by 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay. Cytotoxicity studies revealed that six of the compounds among entire series are three‐fold more potent than the commercially available reference drug tamoxifen against MDA‐MB‐231 and relatively safe towards HEK‐293 cells. In order to validate experimental results, the possible binding interaction of the most potent conjugate and tamoxifen with Topoisomerase II has been scrutinized by molecular docking studies.

2-Benzylidene tetralone derivatives are exhibited as potent anti-breast cancer agents through microtubule stabilization and induction of apoptosis through reactive oxygen species.

In the recent past, various groups have proposed diverse biocompatible methods for the synthesis of metal nanoparticles (NPs). Besides culture biomass, culture supernatants (CS) are increasingly being explored for the synthesis of NPs; however, with the ever-increasing exploration of various CS in the biofabrication of NPs, it is equally important to explore the potential of various culture media (CMs) in the synthesis of metal NPs. Considering these aspects, in the present investigation, we explore the possible applicability of various CMs in the biofabrication of metal NPs.The synthesis of NPs was primarily followed by UV/VIS spectroscopy, and, thereafter, the NPs were characterized by various physiochemical techniques, including EM, EDX, FT_IR, X-ray diffraction, and DLS measurements, and finally, their anticancer potentialities were investigated against breast cancer. In addition, the NPs were examined in conjunction with artemisinin for therapeutic benefits against aggressive and highly metastatic MDA-MB-231 breast cancer cells. Cumulatively, the results of the present study collated the potentials of various bacterial CMs in the biofabrication of metal NPs and ascertained the efficacy of the as-synthesized silver nanoparticles, especially the combinatorial entity as intriguing breast cancer therapeutics. The data of the present study plausibly assist in advancing the therapeutic applicability of the combinatorial amalgam against aggressive and highly metastatic MDA-MB-231 breast cancer cells. K E Y W O R D S artemisinin (ART), breast cancer, culture media (CMs), culture supernatants (CS), nanoparticles (NPs)