The level of production in a variety of organic production systems is often lower than in other traditional production systems. In poultry production, there is also a direct negative effect of the small scale regarding sustainable organic poultry production. Regardless of differences between organic and conventional production systems, this experiment aimed to investigate the usage of tea tree Melaleuca alternifolia (Maiden and Betche) Cheel essential oils as a natural alternative to antibiotics in hen nutrition on productive parameters, table egg quality and eggs fatty acid profile as a promising sustainable organic agricultural tool. A total of 360 Lohmann Brown hens, aged 54 weeks, divided into three different treatment diets, were supplemented with 0 (T1), 40 (T2) and 80 mg/kg (T3) of M. alternifolia essential oil, respectively. Experimental treatments were replicated four times within 30 birds each. The experiment lasted for a total of 56 days (55 to 62 weeks of hens age). A 56-day experimental had two timetable periods of 28 days each: period 1 (55 to 58 weeks of hen age) and period 2 (59 to 62 weeks of hen age). For compound feed supplemented with M. alternifolia essential oil, daily egg production and the efficiency of nutrient utilization (FCR) was improved significantly (p < 0.05) until the end of week 58, with a significant (p < 0.05) increase in the thickness of eggshell, as well as egg production (p < 0.05). However, egg mass, feed consumption, FCR and albumen height, Haugh unit, and eggshell strength did not show any significant (p > 0.05) differences influenced by essential oil feed supplementation. Lower concentrations of saturated fatty acid (SFA) and monounsaturated fatty acid (MUFA), and higher concentrations of polyunsaturated fatty acid (PUFA), were recorded with M. alternifolia essential oil supplementation, but without significant (p > 0.05) differences. At the end of the experiment, the obtained results showed that the addition of M. alternifolia essential oil to hen nutrition had a positive effect on production parameters and eggs fatty acid profile, with increased eggshell thickness (p < 0.05).
The study was aimed at evaluating the presence of antibiotic-resistant Staphylococcus aureus in retailed raw chicken meat from retail stores intended for human consumption. The presence, characterization, and antibiotic susceptibility of S. aureus from 38 retail raw chicken meat samples was performed using a standard microbiological method involving mannitol salt agar (MSA) and Mueller-Hinton agar (MHA). All the samples were positive for Staphylococcus species, of which 34 (89.5%) were positive for S. aureus. The S. aureus isolates were most resistant to tetracycline (88.24%), erythromycin (82.35%), and chloramphenicol (61.77%). Nevertheless, decreased resistance towards gentamycin (23.53%) and cotrimoxazole (38.24%) were recorded. All the S. aureus isolates in this study were resistant to cloxacillin, amoxicillin, and augmentin (amoxicillin + clavulanic acid). The present findings show how the raw chicken meat samples could be a potential source of multidrug-resistant S. aureus strains dissemination. Therefore, this study suggests high-level contamination of meat with multidrug-resistant S. aureus and highlights the public health consequences of consuming such products. Undoubtedly, uncontrolled drugs in food animal production as growth stimulators or medicinal treatment present a possible consequence to people’s health. Having the aforementioned in mind, there is a necessity to control the use of drugs and monitor any residues left in the food intended for human consumption.
This research aimed to determine the prevalence rate of antimicrobial resistance (AMR) of Staphylococcus aureus isolated from raw cow milk samples in Albania and Serbia. A total of 100 raw milk samples, 50 from Albania and 50 from Serbia, have been collected from randomly selected cattle farms. Twelve samples (12 %) were positive for S. aureus, five of those originating from Albania (41.66 %) and seven from Serbia (58.33 %). Resistance of isolated S. aureus strains to ampicillin in milk samples from Albania (36.7 %) and Serbia (34.1 %) was the most common. Some of S. aureus strains from milk samples from Albania were resistant to tetracycline (16.9 %), while isolates from milk samples from Serbia were more resistant towards oxacillin (18 %). The recorded resistance towards erythromycin (13.2 %; 13.1 %), and sulfatrim (7.6 %; 6.9 %) was similar between both milk samples, respectively. The obtained results have shown higher resistance of S. aureus strains towards cephalothin in milk samples from Serbia (3.9 %) compared to milk samples from Albania (1.6 %), respectively. All isolates tested for antibiotic sensitivity were susceptible to methicillin, vancomycin, chloramphenicol, and ciprofloxacin. The obtained results regarding the minimum inhibitory concentrations (MICs) of antibiotics indicated that isolates were resistant to tetracycline, oxacillin, erythromycin, and ampicillin, respectively. Results of our investigation have shown that the found antibiotics residues are related to the usage of erythromycin (72.6 % and 73.9 %), followed by ampicillin (70.4 % and 71.2 %) while residues of ciprofloxacin in the analysed milk samples was not registered.
In June 2014, Albania was granted EU candidate status, thus starting a process of compliance with the membership criteria. In this context, a modern meat inspection approach in line with the European legislation was applied to a pig slaughterhouse in northern Albania in order to investigate the ante-mortem (AM) and post-mortem (PM) conditions and the relationship between these findings. For this purpose, 3930 pigs divided into 35 batches were evaluated over a 3-month period. The most frequent AM conditions recorded were tail lesions and dyspnea (9.1%), followed by skin (8.9%) and ear lesions (8.5%), while in the PM inspections, pleuritis was the most frequently observed condition (10.2%), followed by pneumonia (8.5%), liver alterations (5.7%), milk spot liver (3.8%), and pericarditis (3.3%). With the exception of liver alterations, the other PM lesions mentioned were positively associated with lesions on the ears (OR = 1.036; p < 0.001) and skin (OR = 1.026; p = 0.011) and dyspnea (OR = 1.021; p = 0.005), confirming the link between these variables and the health and welfare conditions of pigs on farms. Overall, the evidence that emerged from this Albanian slaughterhouse can be considered in line with other European contexts, especially in light of the considerable variability in the data present in the literature.
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