The continued monitoring of Echinococcus species in intermediate and definitive hosts is essential to understand the eco-epidemiology of these parasites, as well to assess their potential impact on public health. In Canada, co-infections of Echinococcus canadensis and Echinococcus multilocularis based on genetic characterization have been recently reported in wolves, but not yet in other possible hosts such as coyotes and foxes. In this study, we aimed to develop a quantitative real-time PCR assay to detect E. multilocularis and E. canadensis and estimate the occurrence of co-infections while inferring about the relative abundance of the two parasites within hosts. We tested DNA extracted from aliquots of Echinococcus spp. specimens collected from intestinal tracts of 24 coyote and 16 fox carcasses from Alberta, Canada. We found evidence of co-infections of E. multilocularis and E. canadensis in 11 out of 40 (27%) samples, with 8 out of 24 (33%) in coyote samples and 3 out of 16 (19%) in red fox samples. DNA concentrations were estimated in three samples with Cq values within the range of the standard curve for both parasites; two of them presented higher DNA concentrations of E. multilocularis than E. canadensis. The use of qPCR aided detection of co-infections when morphological discrimination was difficult and quantification of DNA for samples within the standard curve. This is the first molecularly confirmed record of E. canadensis in coyotes and the first evidence of co-infections of E. multilocularis and E. canadensis in coyotes and red foxes.

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The Marbled Murrelet (Brachyramphus marmoratus) is a small seabird that is currently listed as threatened in Canada. Understanding this species’ marine habitat preferences plays a vital role in our ability to focus conservation planning. We used the longest-running at-sea survey dataset available in British Columbia to examine hotspot persistence and habitat use at Laskeek Bay, Haida Gwaii, BC. The Laskeek Bay Conservation Society has been conducting spring and summer surveys along fixed transect routes in open and shoreline waters from 1997 to 2018. Along with analyzing this long-term dataset, we conducted surveys to measure oceanographic variables (2018–2019) and tested whether Marbled Murrelets in the same area used prey and oceanographic information to select marine habitat in conjunction with physical habitat features. Our hotspot persistence map, defined as areas that repeatedly had counts above a 75% threshold relative to other areas during a given survey, showed that murrelets consistently preferred shoreline transects. Murrelets also preferred shallow marine areas closer to streams, above higher proportions of sandy substrate and closer proximity to abundant nesting habitat. Modeling weather and time variables contributed little additional predictive power. Nonetheless, models that included physical environmental, oceanographic, and prey variables outperformed those with only physical environmental variables. Stratified water was the oceanographic variable most strongly related to higher counts. Our study suggests that stratified waters could work with stream systems to create productive zones for foraging murrelets, and highlights the importance of murrelets having access to marine areas with the preferred physical features.

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