The biochemical methane potential (BMP) test was used to evaluate the anaerobic biodegradabilities of food waste (FW), waste activated sludge (WAS), and the mixtures having the ratios of 10:90, 30:70, 50:50, 70:30, and 90:10 (FW:WAS) on a volatile solid (VS) basis. The carbon/nitrogen (C/N) ratio and the biodegradability of the mixtures improved from 6.16 to 14.14 and increased from 36.6 to 82.6% as the FW proportion of the mixture increased from 10 to 90%, respectively. The stability and performance of the single-stage anaerobic digester (SSAD) for the co-digestion of FW and WAS were investigated, operated at the hydraulic retention times (HRTs) of 10, 13, 16, and 20 days with five mixtures at 35 degrees C, respectively. During all the experiments, there were no indication of failure such as low pH, insufficient alkalinity, ammonia inhibition, and the accumulation of volatile fatty acids (VFAs) in any of the digesters, and the buffer capacity was the highest in the digester fed with a feed mixture of 50:50. The optimum operating conditions of the SSAD were found to be an HRT of 13 days and a mixture of 50:50 in terms of the buffer capacity of the digester and the effluent VS concentration, the methane content of the biogas produced and the specific methane production (SMP). The VS removal efficiency, biogas production rate (GPR), and SMP in this condition achieved 56.8%, 1.24 m3 m(-3) d(-1) and 0.321 m3 CH4 kg(-1) VS(fed)(-1) with an organic loading rate (OLR) of 2.43 kg VS m(-3) d(-1).
Ethanol production from concentrated oak wood hydrolysate was carried out to obtain a high ethanol concentration and a high ethanol yield. The effect of added inhibitory compounds, which are typically produced in the pretreatment step of steam-explosion on ethanol fermentation, was also examined. p-Hydroxybenzoic aldehyde, a lignin-degradation product, was the most inhibitory compound tested in this study. Compounds with additional methyl groups had reduced toxicity and the aromatic acids were less toxic than the corresponding aldehydes. The lignin-degradation products were more inhibitory than the sugar-derived products, such as furfural and 5-hydroxymethylfurfural (HMF). Adaptation of yeast cells to the wood hydrolysate and detoxification methods, such as using charcoal and overlime, had some beneficial effects on ethanol production using the concentrated wood hydrolysate. After treatment with charcoal and low-temperature sterilization, the yeast cells could utilize the concentrated wood hydrolysate with 170 as well as 140 g/L glucose, and produce 69.9 and 74.2 g/L ethanol, respectively, with a yield of 0.46-0.48 g ethanol/g glucose. In contrast, the cells could not completely utilize untreated wood hydrolysate with 100 g/L glucose. Low-temperature sterilization, with or without charcoal treatment, was very effective for ethanol production when highly concentrated wood hydrolysates were used. Low-temperature sterilization has advantages over traditional detoxification methods, such as using overlime, ion exchange, and charcoal, because of the reduction in the total cost of ethanol production.
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