No abstract

Purpose -This paper aims to define the effect of specimen mesostructure on the monotonic tensile behavior and tensile-fatigue life of layered acrylonitrile butadiene styrene (ABS) components fabricated by fused deposition modeling (FDM). Design/methodology/approach -Tensile tests were performed on FDM dogbone specimens with four different raster orientations according to ASTM standard D638-03. Resulting ultimate tensile stresses (UTS) for each raster orientation were used to compute the maximum stress for fatigue testing, i.e. 90, 75, 60 and 50 or 45 per cent nominal values of the UTS. Multiple specimens were subjected to tension -tension fatigue cycling with stress ratio of R ϭ 0.10 in accordance with ASTM standard D7791-12. Findings -Both tensile strength and fatigue performance exhibited anisotropic behavior. The longitudinal (0°) and default (ϩ45/Ϫ45°) raster orientations performed significantly better than the diagonal (45°) or transverse (90°) orientations in regards to fatigue life, as displayed in the resulting Wohler curves. Practical implications -Raster orientation has a significant effect on the fatigue performance of FDM ABS components. Aligning FDM fibers along the axis of the applied stress provides improved fatigue life. If the direction of applied stresses is not expected to be constant in given application, the default raster orientation is recommended. Originality/value -This project provides knowledge to the limited work published on the fatigue performance of FDM ABS components. It provides S-N fatigue life results that can serve as a foundation for future work, combining experimental investigations with theoretical principles and the statistical analysis of data.

Non-invasive techniques for quantifying early biochemical and biomechanical changes in articular cartilage may provide a means of more precisely assessing osteoarthritis (OA) progression. The goals of this study were to determine the relationship between T1rho magnetic resonance (MR) imaging relaxation times and changes in cartilage composition, cartilage mechanical properties, and synovial fluid biomarker levels and to demonstrate the application of T1rho imaging to evaluate cartilage composition in human subjects in vivo. Femoral condyles and synovial fluid were harvested from healthy and OA porcine knee joints. Sagittal T1rho relaxation MR images of the condyles were acquired. OA regions of OA joints exhibited an increase in T1rho relaxation times as compared to non-OA regions. Furthermore in these regions, cartilage sGAG content and aggregate modulus decreased, while percent degraded collagen and water content increased. In OA joints, synovial fluid concentrations of sGAG decreased and C2C concentrations increased compared to healthy joints. T1rho relaxation times were negatively correlated with cartilage and synovial fluid sGAG concentrations and aggregate modulus and positively correlated with water content and permeability. Additionally, we demonstrated the application of these in vitro findings to the study of human subjects. Specifically, we demonstrated that walking results in decreased T1rho relaxation times, consistent with water exudation and an increase in proteoglycan concentration with in vivo loading. Together, these findings demonstrate that cartilage MR imaging and synovial fluid biomarkers provide powerful non-invasive tools for characterizing changes in the biochemical and biomechanical environment of the joint.

Severe obesity increased load-induced cartilage damage, while milder changes in adiposity/metabolic syndrome seen in TLR5KO mice did not. Furthermore, the effects of systemic inflammation/obesity on cartilage damage depend on the duration of mechanical loading. Lastly, reduced cartilage damage in the TLR5KOΔMicrobiota mice suggests that the gut microbiome may influence cartilage pathology.

Posttraumatic osteoarthritis (PTOA) is associated with abnormal and increased subchondral bone remodeling. Inhibiting altered remodeling immediately following joint damage can slow PTOA progression. Clinically, however, inhibiting remodeling when significant joint damage is already present has minimal effects in slowing further disease progression. We sought to determine the treatment window following PTOA initiation in which inhibiting remodeling can attenuate progression of joint damage. We hypothesized that the most effective treatment would be to inhibit remodeling immediately after PTOA initiation. We used an animal model in which a single bout of mechanical loading was applied to the left tibia of 26‐week‐old male C57Bl/6 mice at a peak load of 9 N to initiate load‐induced PTOA development. Following loading, we inhibited bone remodeling using daily alendronate (ALN) treatment administered either immediately or with 1 or 2 weeks' delay up to 3 or 6 weeks post‐loading. A vehicle (VEH) treatment group controlled for daily injections. Cartilage and subchondral bone morphology and osteophyte development were analyzed and compared among treatment groups. Inhibiting remodeling using ALN immediately after load‐induced PTOA initiation reduced cartilage degeneration, slowed osteophyte formation, and preserved subchondral bone volume compared to VEH treatment. Delaying the inhibition of bone remodeling at 1 or 2 weeks similarly attenuated cartilage degeneration at 6 weeks, but did not slow the development of osteoarthritis (OA)‐related changes in the subchondral bone, including osteophyte formation and subchondral bone erosions. Immediate inhibition of subchondral bone remodeling was most effective in slowing PTOA progression across the entire joint, indicating that abnormal bone remodeling within the first week following PTOA initiation played a critical role in subsequent cartilage damage, subchondral bone changes, and overall joint degeneration. These results highlight the potential of anti‐resorptive drugs as preemptive therapies for limiting PTOA development after joint injury, rather than as disease‐modifying therapies after joint damage is established. © 2021 American Society for Bone and Mineral Research (ASBMR).