This study aimed to assess an in vitro antioxidant and hemolytic activities of crude hydromethanolic extract, ethyl acetate and n-butanol fractions from leaves and stems of Salvia officinalis L. Extract and fractions prepared by maceration from S. officinalis were determined for their phytochemical composition and their contents in total phenolic and flavonoid. Antioxidant activity was evaluated by the methods of DPPH radical scavenging, ferric reducing antioxidant power and total antioxidant capacity. Hemolysis assay was carried out to evaluate the toxicity of the studied samples. Crude extract and fractions from S. officinalis contain different secondary metabolites and considerable contents of phenolic and flavonoids. They exhibit high antioxidant activity and low hemolytic effect. However, ethyl acetate fraction is characterized by the highest total phenolic (362.75 ± 0.07 µg GAE/mg DE) and flavonoid (263.27 ± 0.1 µg CE/mg DE) contents. Furthermore, this fraction shows the best antioxidant activity tested by DPPH, FRAP and TAC assays. The antiradical activity of ethyl acetate fraction against DPPH (IC50 = 208.51 ± 5.77 μg/mL) is close to that of ascorbic acid (IC50 = 206.43 ± 4.16 μg/mL). It exhibits a powerful activity to reduce iron (EC50 = 250 ± 0.001 μg/mL) and the best total antioxidant capacity (817.33 ± 0.06 μg/mL). It reveals the lowest hemolysis rate of 3.77 ± 0.02% with 200 mg/mL of fraction, after one hour of incubation. The obtained results suggest that crude extract, ethyl acetate and n-butanol fractions from Salvia officinalis L. are considered efficient natural antioxidants and safe for human erythrocytes. REFERENCES: Andrei, M., Cadmiel, M., Simone, C., Marcello, L., Marina, S., & Gianina, C. (2018).Determination of phenolic compounds in different species of salvia from Romania(Salvia transsylvanica, Salvia glutinosa, Salvia officinalis) and their biologicalactivities. 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This research aims to reveal the level of Islamic behavior and the quality of the performance of human resources at the Zakat Fund in Lebanon - Dar Al Fatwa, from the point of view of human resources who were estimated at 125 individuals who served in the institution during the period of obtaining the ISO international quality certificate and to compare the results with some partial studies. A questionnaire was designed and analyzed. It expresses the level of Islamic behavior through its four domains related to its ethical system represented in behavior towards the Almighty God, towards the self, and towards others. Its dimensions were chosen through politeness with God Almighty, integrity, humility, and environmental behavior, within the framework of three main axes that are the work environment, the personal environment and Islamic behavior training. The quality of human resources performance has been measured through the dimensions of the ServQual scale, which expresses the quality of performance. The SPSS program was used to analyze the respondents' estimates. This research clarifies the Islamic view on the necessity to balance behavior and not to contradict it between the work environment, and the personal environment, and the importance of behavioral training. It also points out the need to work on standards of behavior in order to focus on improving performance. This paper attempts to provide a measure to verify the level of quality of performance, and the level of Islamic behavior in line with Islam in its behavioral view that stresses the homogeneity of behavior in the workplace, the personal environment, and behavioral training. This measure will support the civilized view of Islam and its institutions and alleviate Islamophobia.
The consumer necessity for a good quality of olive oil in terms of its lipid and phenolic profiles is demanded to preserve a good health and to combat illnesses. The conformity of olive oil with the International Olive Council standards requires the determination of certain chemical parameters including free acidity, saponification and peroxide indices. Our study was based on a comparative evaluation of chemical quality indices and extraction of phenolic compounds from different samples of olive oil of different durations of storage, from different regions of Algeria and with different preparations. The most recent olive oil sample presented the least acidity (1.88%) and saponification index (185.32 mg of KOH/g of oil). However, the sample conserved for five years revealed the highest quantities of total phenolics (0.56 ± 0.02 mg GAE/g oil) and flavonoids (0.174 ± 0.019 mg CE/g oil). Different results were collected comparing samples of olive oil from different regions from Algeria. Acidity percentages were ranged from 0.7 to 3.1%, peroxide index from 8 to 53meqO2/Kg of oil and saponification number from 166.61 to 201.11 mg KOH/g of oil. Yields of phenolic extraction results revealed percentages from 0.11 to 0.24%. The traditionally prepared olive oil presented the best quality with an acidity of 1.01% and a saponification number of 187.10 ± 13.68 mg KOH/g oil. Whereas, the industrially prepared one exhibited the lower number of peroxide with 8.30 ± 2.88 meqO2/ kg oil. The quality of olive oil was influenced by time, geographical region and mode of extraction. Conserved olive oils may be destined for the manufacture of soaps or the extraction of phenolic compounds
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