Antioxidant Activity and Hemolytic Effect of Hydro-methanolic Extract and its Phenolic Enriched Fractions From Leaves and Stems of Salvia officinalis L. from Algeria

Abstract

This study aimed to assess an in vitro antioxidant and hemolytic activities of crude hydromethanolic extract, ethyl acetate and n-butanol fractions from leaves and stems of Salvia officinalis L. Extract and fractions prepared by maceration from S. officinalis were determined for their phytochemical composition and their contents in total phenolic and flavonoid. Antioxidant activity was evaluated by the methods of DPPH radical scavenging, ferric reducing antioxidant power and total antioxidant capacity. Hemolysis assay was carried out to evaluate the toxicity of the studied samples. Crude extract and fractions from S. officinalis contain different secondary metabolites and considerable contents of phenolic and flavonoids. They exhibit high antioxidant activity and low hemolytic effect. However, ethyl acetate fraction is characterized by the highest total phenolic (362.75 ± 0.07 µg GAE/mg DE) and flavonoid (263.27 ± 0.1 µg CE/mg DE) contents. Furthermore, this fraction shows the best antioxidant activity tested by DPPH, FRAP and TAC assays. The antiradical activity of ethyl acetate fraction against DPPH (IC50 = 208.51 ± 5.77 μg/mL) is close to that of ascorbic acid (IC50 = 206.43 ± 4.16 μg/mL). It exhibits a powerful activity to reduce iron (EC50 = 250 ± 0.001 μg/mL) and the best total antioxidant capacity (817.33 ± 0.06 μg/mL). It reveals the lowest hemolysis rate of 3.77 ± 0.02% with 200 mg/mL of fraction, after one hour of incubation. The obtained results suggest that crude extract, ethyl acetate and n-butanol fractions from Salvia officinalis L. are considered efficient natural antioxidants and safe for human erythrocytes.
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