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In the current study we had developed and characterized Poly (lactic-co-glycolide) acid nanoparticles for anticancer drug Doxorubicin and further studied in-vitro cytotoxic effect of free and encapsulated nanoparticle drug delivery system. PLGA-drug nanoparticles were prepared by oil-water emulsion solvent evaporation method. The morphology of drug loaded nanoparticles was analyzed using a scanning electron microscope. The drug loaded nanoparticles were evaluated for cell cytotoxicity by sulforhodamine B assay. Images of formulation indicate the surface morphology of formulation. The in vitro cytotoxicity results showed Doxorubicin NPs to be active against A431 and NCI-H322 cell line compared to Doxorubicin free drug. The IC50 values were found to be 1.73 µM with A431 cell line whereas 1.64 µM for NCI-H322 cell line. This indicates that Doxorubicin NPs have more pronounced activity towards A431cell line.

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