Present study aims to evaluate phytochemical and chromatographic profile, along with antioxidant and anti proliferative properties of Mimosa diplotricha ethanolic leaf extract. Qualitative screening of phytoconstituents by consecutive solvent extraction in increased polarity basis and standardization of potential extract based on phytochemical elution profile was done. Selected secondary metabolites like phenols, flavanoids, tannins and alkaloids were quantified in ethanolic extract. Chromatographic profile was determined by HPTLC and GC-MS analysis. In vitro antioxidant potential was assessed by DPPH, superoxide, nitric oxide, hydrogen peroxide and hydroxyl radical scavenging assay. Ferric-reducing antioxidant power assay (FRAP) and reducing potential of the respective extract were also determined. Anticancer potential was confirmed by cytotoxic screening in colorectal (HCT-116) cancer cell lines by MTT assay. Qualitative phytochemical analysis and chromatographic profile reveal a phytoconstituent rich profile for the ethanolic leaf extract. The amount of, phenols (56 ± 0.57 mg/g), flavanoids (27 ± 0.76 mg/g), tannins (33 ± 0.15 mg/g) were quantified as equivalent of gallic acid, quercetin and tannic acid standards respectively and alkaloids (2.51 ± 0.47 mg/g of extracted plant material) were expressed based on respective analysis. Results also reveal convincing antioxidant potential for respective extract. In vitro cytotoxicity confirmed by MTT assay represents an IC50 value of 97.82 µg/ml. From the above results it can be concluded that M.diplotricha has got pharmacologically significant phytoconstituents and therapeutic active ingredients as evident in HPTLC and GC-MS analysis. This is further supported by considerable antioxidant and anti proliferative properties observed in respective assays.

Quantitative and qualitative analysis of different components and antioxidant and activities of the extract of flower (CPF) were analyzed in . identification of of flower was also identified by GC-MS analysis. assess the biochemical features of CPF. solvent extraction of CPF was performed using solvents in increasing order of polarity (petroleum ether, chloroform, ethyl acetate, ethanol, and water) and solvent with maximum profile was standardized for further analysis. Quantitative analysis of selected secondary metabolites like tannin, , alkaloids, and of the flower extract was done by UV . In antioxidant assays and in efficacy of the flower extract were analyzed by respective in assays. identification of in CPF was identified by using GC-MS analysis revealed secondary metabolites in the extract, and further analysis of the extract was performed. Quantitative estimation revealed an accountable amount of secondary metabolites like (47.66mg/g acid equivalent), (24mg/g equivalent), (41mg/g equivalent), and alkaloids (1.79mg/g of extracted plant material). analysis (GC-MS) also confirmed convincing compounds in the extract. From in antioxidant and assay, the IC50 value of the extract of CPF was measured and compared with standard, and from the results, it was evident that the extract had significant in antioxidant and activity. the above results, it can be confirmed thatCPF has got significant and therapeutically active ingredients, as evident in analysis. This is further supported by considerable antioxidant and properties observed in respective assays.

Jain et al.: Biological aspect of Thymus vulgarisThymus vulgaris L. commonly known as thyme or garden thyme belonging to family Lamiaceae is a pleasant smelling flowering perennial shrub, cultivating all over the world. Thyme has great importance due to the possibility of its use in various applications, as food additives, in medicines and in cosmetic industry. Thyme leaves are highly aromatic therefore commonly used fresh or dried as a seasoning in a variety of culinary applications including soups, stews, sauces, meat, fish dishes and for flavoring liqueurs, herbal tea preparations. Thymus is an important medicinal plant, highly endorse due to a broad range of therapeutic properties of their chemical components such as antirheumatic, antidermatophytic, antioxidants, antiseptic, antispasmodic, antimicrobial, cardiac, carminative, astringent, diuretic and expectorant. The plant is also useful against cough, cold, chest infections, diabetes and for digestive upset. Flavonoids have good potential as antioxidants and antifungal behavior. The present review article gives comprehensive information about various medicinal and traditional utility and pharmacological activities of the thymus plant and its constituents.

Aim: To study the pretreatment effect of ethanolic extract of Azolla microphylla (EAM) on rat liver induced with Isoproterenol (ISO) and to identify the phytochemicals present in EAM using HPTLC and GCMS techniques. Materials and Methods: 42 male Wistar rats were divided into 7 groups. Rats were pre-treated with EAM (250 and 500 mg/kg bw) orally for 28 days and induced with ISO (85 mg/kg; intra-peritoneal) on the 29th and 30th days. Blood and liver samples were collected from all the animals on 30th day for biochemical and histopathological observations. HPTLC and GC-MS analysis of EAM were done using the standard protocols. Results: The ISO-induced group of rats displayed significant decrease in the hepatic tissue level and activities of total protein and aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP) enzymes, respectively. Moreover, a significant decline in enzymatic and non-enzymatic antioxidants levels was spotted in the same group. However, EAM pretreatment for 28 days significantly protected the rat liver from the aforementioned alterations. Nevertheless, histopathological analysis revealed central vein dilation, necrosis, and infiltration of inflammatory cells in the ISO-induced group, wherein, EAM pretreatment significantly protected the hepatocytes from the above-mentioned changes indicating its antioxidant and cytoprotective potential. HPTLC analysis displayed the presence of flavonoids. The GC-MS analysis confirmed the presence of quercetin in EAM. Conclusion: The overall results suggest that EAM pretreatment possesses ameliorative effect against the ISOinduced oxidative damage in the rat hepatocytes.

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