An incision is an injury caused by direct contact of the body with sharp objects. Cassava leaves (Manihot esculenta Crantz) are a medicinal plant known to contain compounds that play a role in wound healing, such as vitamin C, flavonoids, triterpenoids, tannins and saponins. This study aims to determine the effect of ethanol extract of cassava leaves on the healing of cuts in rabbits. The ethanol extract of cassava leaves was prepared by maceration using 70% ethanol as a solvent. The wound healing test in rabbits was divided into 5 treatment groups, namely the positive control, the negative control, and test groups were given ethanol extract of cassava leaves concentration of 5%, 10% and 20%. Making an incision using a scalpel which is sliced into the back of the rabbit for 5 incisions. Rabbits were observed every day for one week with statistical analysis One Way ANOVA test followed by LSD test with Post Hoc Test. The ethanol extract of cassava leaves with a concentration of 5% 10% and 20% has the effect of wound healing.The concentration of 5% is (9.71 ± 0.17)%, the concentration of 10% is (17.19 ± 0.05)%, and a concentration of 20% is (23.24 ± 0.04)%. The results of the LSD test with the Post Hoc Test showed that there was no significant difference between the ethanol extract of 20% cassava leaves and the positive control, which means that the ethanol extract of cassava leaves can be used as wound healing in rabbits.