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Molecular allergy diagnosis allows to identify sensitization to a large range of respiratory allergens, which is important in the diagnosis of allergic asthma. Moreover, based on the reactive IgE profile detected at an early age, it is possible to predict the development of allergic respiratory diseases even at the preclinical stage. The goal of the pilot project was to create a children's passport sensitization using a multiplex panel to be able to identify groups at risk for the development of asthma in children of early school age. Materials and methods. Parents of 33 students studying in the second grade of the Tashkent gymnasium were questioned using the ISAAC questionnaire for the clinical manifestations of respiratory diseases in their children. Their children’s sera were tested for the presence of IgE to various allergenic molecules using the MeDALL research method. Results. 30% of children were sensitized to at least one allergen, and 30% of them were born by Caesarean section. and 30% of them were born by Caesarean section. In total, 68 of the 176 allergenic molecules located on the MeDALL microchip were recognized. 80% of sensitized children had a genuine polysensitization to 3 or more major allergens. In 18% of children, IgE to household molecules of the risk of developing asthma, such as Alt a 1, Bla g 1, Bla g 2, Can f 1, Fel d 1, were detected. Conclusions. In general, clinical manifestations in the form of wheezing and obstruction were detected in 60% of sensitized children, but only 40% of these children were under the supervision of an allergist or received symptomatic treatment. In 20% of already sensitized children, clinical symptoms were absent. Molecular testing of the profile of IgE sensitization and the creation of an allergic passport allows us to identify a group of children at risk for developing asthma. This information can be used to assign therapy and secondary prevention of asthma in the early school years.

Молекулярная аллергодиагностика позволяет выявлять сенсибилизацию к большому спектру респираторных аллергенов, что является важным в диагностике аллергической астмы. Более того, на основании IgEреактивного профиля, выявляемого в раннем возрасте, можно прогнозировать развитие аллергических респираторных заболеваний еще на доклиническом этапе. Целью пилотного проекта явилось создание паспорта сенсибилизации школьника с использованием мультиплексной панели для возможности выявления группы риска по развитию астмы у детей раннего школьного возраста. Материалы и методы. Родители 33 учеников, обучающихся во втором классе гимназии города Ташкента, были опрошены по вопроснику ISAAC на наличие клинических проявлений респираторных заболеваний у их детей. Сыворотки крови их детей были протестированы на наличие IgE к различным аллергенным молекулам при помощи исследовательского аллергочипа MeDALL. Результаты. 30 детей были сенсибилизированы как минимум к одному аллергену, и из этого числа 30 родились путем операции кесарева сечения. Всего было распознано 68 аллергенных молекул из 176, расположенных на микрочипе MeDALL. 80 сенсибилизированных детей имели подлинную полисенсибилизацию к 3 и более мажорным аллергенам. У 18 детей были выявлены IgE к бытовым молекулам риска развития астмы, таким как Alt a 1, Bla g 1, Bla g 2, Can f 1, Fel d 1. Заключение. В целом клинические проявления в виде хрипов и обструкции были выявлены у 60 сенсибилизированных детей, но только 40 таких детей находились под наблюдением аллерголога или получали симптоматическое лечение. У 20 уже сенсибилизированных детей клиническая симптоматика отсутствовала. Молекулярное тестирование профиля IgEсенсибилизации и создание аллергопаспорта позволяют определить группу детей с риском развития астмы. Данная информация может быть использована для своевременного назначения терапии и вторичной профилактики астмы уже в ранние школьные годы.Molecular allergy diagnosis allows to identify sensitization to a large range of respiratory allergens, which is important in the diagnosis of allergic asthma. Moreover, based on the reactive IgE profile detected at an early age, it is possible to predict the development of allergic respiratory diseases even at the preclinical stage. The goal of the pilot project was to create a childrens passport sensitization using a multiplex panel to be able to identify groups at risk for the development of asthma in children of early school age. Materials and methods. Parents of 33 students studying in the second grade of the Tashkent gymnasium were questioned using the ISAAC questionnaire for the clinical manifestations of respiratory diseases in their children. Their childrens sera were tested for the presence of IgE to various allergenic molecules using the MeDALL research method. Results. 30 of children were sensitized to at least one allergen, and 30 of them were born by Caesarean section. and 30 of them were born by Caesarean section. In total, 68 of the 176 allergenic molecules located on the MeDALL microchip were recognized. 80 of sensitized children had a genuine polysensitization to 3 or more major allergens. In 18 of children, IgE to household molecules of the risk of developing asthma, such as Alt a 1, Bla g 1, Bla g 2, Can f 1, Fel d 1, were detected. Conclusions. In general, clinical manifestations in the form of wheezing and obstruction were detected in 60 of sensitized children, but only 40 of these children were under the supervision of an allergist or received symptomatic treatment. In 20 of already sensitized children, clinical symptoms were absent. Molecular testing of the profile of IgE sensitization and the creation of an allergic passport allows us to identify a group of children at risk for developing asthma. This information can be used to assign therapy and secondary prevention of asthma in the early school years.