No abstract

Devices in current use for adsorptive plasma treatment have, in many cases, been adapted from other types of separation processes. Minimizing system volume and quantity of ligand per mass transferred are proposed as appropriate design goals for an adsorptive plasma treatment system. The process consists of two operations: cell/plasma separation and solute adsorption/desorption. An example of an optimally designed membrane cell separator is presented which adds a negligibly small volume to the system. By overcoming mass transfer limitations, both volume and ligand quantity associated with the sorbent device can be minimized. Combining both of the above operations in a single device is also feasible. Examples are discussed of optimally designed sorbent devices and devices which combine cell separation/solute adsorption.

A multipurpose fluidized-bed receptor-affinity purification system based upon the biological recognition between an immobilized receptor and its soluble protein ligands is described. The fluidized affinity sorbent consists of a soluble form of interleukin-2 receptor chemically bonded to an aldehyde derivative of controlled pore glass beads, which have a pore diameter of 1000 A and a particle density of 1.2-1.3 g/mL. The fluidized-bed separation device used in this study consists of a specially designed column fitted at the inlet end with a perforated distributor plate covered with a screen and the top outlet with an adjustable piston. The fluidized-bed consisting of a loose gel matrix permits the unimpeded passage of cell debris and particulate matter, while the target protein is captured by the affinity beads. Purification of the humanized-anti-Tac monoclonal antibody is used as a model system to determine the operational parameters. Also, fluidized-bed receptor-affinity chromatography has been successfully employed in the purification of recombinant interleukin-2 and single chain anti-Tac(Fv)-Pseudomonas exotoxin immunotoxin from unclarified inclusion body extracts. Overall, fluidized-bed receptor-affinity chromatography is found to be a productive affinity method suitable for the purification of recombinant human interleukin-2 and related molecules.

No abstract

No abstract