Membrane-Based Affinity Technology for Commercial Scale Purifications

An Introduction to Monolithic Disks as Stationary Phases for High Performance Biochromatography

“…About ten years ago, it was realized that the selectivity of microfilter membranes could be increased dramatically by the introduction of interactive ionic, hydrophobic, and especially, biospecific groups on the (pore) surface [2]. Through this, the membrane became what at that time was called a membrane absorber [3 -7].…”

Preparation and performance of cellulose acetate/polyethyleneimine blend microfiltration membranes and their applications

“…Adsorptive membrane substrates should be mechanically resilient and resistant to solvents used to activate coupling and not participate in secondary hydrophobic adsorption, which produces non-specific retention that interferes with product resolution or can lead to the denaturation of biopolymers. Most of the polymers used in manufacturing of microfiltration membranes, such as nylon [10][11][12], polysulfone [13,14] or polyethylene [15], display undesirable surface characteristics leading to strongly non-specific adsorption of proteins on the membrane surfaces. So it is needed to further modify the membranes to improve hydrophilicity of their surface and reduce the non-selective adsorption of proteins.…”

Proceedings: The Multidisciplinary Approach to Develop Artificial Organs

“…If it is too short, a significant portion of the solute will remain unbound and exit the column before the ligand sites are saturated. The relationship between the time needed for diffusion to the bead core, t D , and the residence time of the solution on the column, t C , was given by Brandt et al [10] in terms of the Peclet number:…”