Data generation is no longer the limiting factor in advancing biological research. In addition, data integration, analysis, and interpretation have become key bottlenecks and challenges that biologists conducting genomic research face daily. To enable biologists to derive testable hypotheses from the increasing amount of genomic data, we have developed the VirtualPlant software platform. VirtualPlant enables scientists to visualize, integrate, and analyze genomic data from a systems biology perspective. VirtualPlant integrates genome-wide data concerning the known and predicted relationships among genes, proteins, and molecules, as well as genome-scale experimental measurements. VirtualPlant also provides visualization techniques that render multivariate information in visual formats that facilitate the extraction of biological concepts. Importantly, VirtualPlant helps biologists who are not trained in computer science to mine lists of genes, microarray experiments, and gene networks to address questions in plant biology, such as: What are the molecular mechanisms by which internal or external perturbations affect processes controlling growth and development? We illustrate the use of VirtualPlant with three case studies, ranging from querying a gene of interest to the identification of gene networks and regulatory hubs that control seed development. Whereas the VirtualPlant software was developed to mine Arabidopsis (Arabidopsis thaliana) genomic data, its data structures, algorithms, and visualization tools are designed in a species-independent way. VirtualPlant is freely available at www.virtualplant.org.

A 28-year-old submarine officer was involved in a dysbaric exposure at 155 feet for 21 hours. Subsequent to rescue, he developed a painful osteonecrosis involving over two-thirds of the femoral shaft and distal metaphysis. Pain is totally atypical for a bony injury of this distribution but because of failure to improve after 12 months, he underwent a series of three operations: (1) core biopsy, (2) intramedullary reaming and intramedullary rod fixation of the femur, and (3) bone grafting to a metaphyseal defect in the distal femur. Serial MRI scans were used to follow the progression of his osteonecrosis. This case report documents the first reported dysbaric diaphyseal osteonecrosis requiring surgery and intramedullary fixation to obtain a satisfactory clinical result.