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Two green algae (Ulva rigida and Cladophora coelothrix), the mussel Mytilus galloprovincialis and the snail Rapana thomasiana from the Bulgarian Black Sea shore have been treated with diesel fuel (100 mg l-1) in an aquarium with sea-water for three days. The lipids and their fatty acid changes have been examined. Significant changes have been observed mainly in the polar lipids and in the saturation of the fatty acids. These changes appeared to be bigger in the evolutionary less advanced species from both groups of marine organisms D algae and invertebrates (Ulva rigida and Mytilus galloprovincialis respectively). The data obtained could be used for a biomonitoring of the pollution

Eighteen sterols were identified in Botryllus schlosseri, stanols being the main sterols. The sterol composition is in agreement with our recent paper on taxonomic separation of tunicates into three groups. Again we found in invertebrates of the Black Sea sterols with a (22Z)-double bond. This confirms the presence of such sterols in nature. The composition of the phospholipids appeared to be complex, and twelve groups of them were identified in Botryllus schlossen. The main representatives of phospholipids appeared to be phosphatidylcholine and phosphatidylethanolamine. Only four volatile compounds were identified, which is unusual for marine invertebrates.