A phenol aqueous emulsion allows the reassociation of DNA at temperatures from 6 to 68 °C. This phenol emulsion reassociation technique (PERT) also promotes the very rapid reassociation of DNA. E. coli and human DNAs at a concentration of 4 ng/mL reacted at room temperature with the PERT reassociate many thousand times faster than under the standard conditions of 0.18 M Na+, 60 °C. Solutions of DNA ranging in concentration from 6 X 10-5 to 6400 yug/mL have been successfully reassociated using the emulsion method. The greatest rate increases are seen at low DNA concentration. The half-time of reassociation does not decrease proportionally with an increase in DNA concentration when using the PERT. At 6400 Mg/mL the phenol emulsion rate of reassociation is only about 10 times faster than under the standard aqueous reference conditions of 0.12 M phosphate buffer (PB), 60 °C. The rate of DNA reassociation observed with the emulsion technique is at least dependent on: (a) the presence of an emulsion; (b) the type and concentration of ion present; (c) an appropriate temperature of incubation; (d) the proper pH; (e) the rate and manner of agitating the emulsion;

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