Methicillin resistant Staphylococcus aureus (MRSA) is a growing concern in the community and in hospitals ever since it has been diagnosed. As staphylococcus has been the dominant pathogen of wounds, Methicillin resistance made the treatment a tough challenge to the clinician. The growing problem of antibiotic resistance and the need for alternative treatments have led to a reappearance of importance in phage therapy. Scope: The present study aimed on MRSA phage types and its antibiotic susceptibility pattern in wounds. Material and Methods: In this study a total of 92 coagulase positive Staphylococcus aureus obtained from various wounds are identified by inoculating on Blood agar, MacConkey agar and Nutrient agar and processed further by necessary biochemical tests and tube coagulase tests. All strains were tested for Methicillin resistance and phage typing. Findings: The results obtained were MRSA (22) of 23.92%, Methicillin sensitive staphylococcus aureus (MSSA) (70) of 76.08% and the predominant phage type in MRSA and MSSA obtained was phage III. The typable MRSA strains and MSSA strains showed 100% sensitivity to Vancomycin followed by 89.13% sensitivity to Cefaperazone-Sulbactum, 87.5% to Ciprofloxacin. Conclusion: Phage typing may be a very useful diagnostic method in future.

Acute lower respiratory tract infections (LRTs) are the leading cause of mortality in below 5 year aged children and shows higher incidence among low income group. Scope: The present study was conducted on one hundred twenty paediatric patients suffering from acute lower respiratory tract infections were evaluated. Lung aspirates, blood samples and throat swabs were investigated bacteriologically to identify the prevalent bacterial isolates and their susceptibility patterns. This study observed that acute LRTIs were predominant among males, effects especially below 5 years aged children. Material and Methods: Lung aspirates were collected under the guidance of paediatrician and processed on sheep Blood agar, Chocolate agar, Nutrient agar and MacConkey’s agar followed by incubated for 18-24hrs, simultaneously the blood samples and throat samples were inoculated in appropriate culture media and processed for pathogens. Findings: Bacteriological analysis revealed 47.5 %(57) positivity in lung aspirates, 15.8% (19) positivity in blood culture and 7.5 %(9) positivity for pathogenic organisms in throat swabs. The dominant organism remained Streptococcus pneumoniae followed by Hemophilus influenza, Klebsiella pneumonia. All the pathogens were subjected to antibiotic sensitivity testing by Modified Kirby-Bauer’s method and tested for Meropenem, Ampicillin, Cotrimoxazole, Ciprofloxacin, Cefotaxime, and Amikacin and observed maximum sensitivity to parenteral drugs such as Carbapenems, Cefotaxime and Aminoglycosides. Conclusion: Comparative study with different samples provides statistically significant values.