Snake fruit is a tropical fruit that is currently in great interest by the public. The This fruit is known to have high antioxidants so it can prevent an increase in cholesterol and other plasma lipids. Moreover, it can also prevent diarrhea. This study investigated the effective which one of the effective doses (25%, 50%, and 100%) from snake fruit extract against the ESBL-Escherichia coli (E. coli). This study was an experimental study with a post-test-only control group design in Microbiology Laboratory, Universitas Prima Indonesia. To investigate the antibacterial activity of snake fruit extract, this study was used disc diffusion methods which used five different groups including positive control (meropenem), negative control (DMSO), 100%, 50%, and 25% of snake fruit extract. The antibacterial assay showed that it required 100% snake fruit extract to inhibit the growth of ESBL-E. coli with average inhibition zone diameter of 7.33 ± 0.58 mm. The lower concentration extract did not show any antibacterial activity like the negative control group. The positive control group showed the most potent antibacterial activity with an average inhibition zone diameter of 36.33 ± 0.58 mm. Thus, it can be concluded that the snake fruit has antibacterial activity against ESBL-E. coli, but it was not as good as the meropenem

Bacillus cereus is aerobic, positive gram, and spore-forming bacilli bacteria. The enterotoxin of this bacteria can cause food poisonous that manifest as diarrhea and vomitus. Some previous study has been performed to explore the antibacterial effect of Indian borage leaf, but none of them were explore the antibacterial effect of Indian borage against Bacillus cereus. Hence this study was design to explore the antibacterial activity of ethanol extract of Indian borage against Bacillus cereus. This was an experimental study with post only control group design. Ethanol extract was extracted by maceration methods and antibacterial activity against Bacillus cereus was evaluated by disc diffusion methods. The obtained ethanol extract was dissolved into some concentraions (1 g/ml, 0.8 g/ml, 0.6 g/ml, 0.4 g/ml, 0.2 g/ml). Data was analysed by One Way ANOVA and followed by Post hoc Test Tukey HSD using SPSS 25. Ethanol extract of Coleus amboinicus showed significant difference at the two highest concentration group against the lowest concentration (P- Value < 0.05). The average of inhibition zone diameter from the lowest (0.2 gr/ml) and highest (1.0 gr/ml) concentration were 14.87 mm and 31.50 mm, respectively. Overall, ethanol extract of Indian borage leaves had potential antibacterial activity against Bacillus cereus. This antibacterial activity increase followed by the increase of the concentration.