The microbial community dynamics of a functionally stable, well-mixed, methanogenic reactor fed with glucose were analyzed over a 605-day period. The reactor maintained constant pH and chemical oxygen demand removal during this period. Thirty-six rrn clones from each of seven sampling events were analyzed by amplified ribosomal DNA restriction analysis (ARDRA) for the Bacteria andArchaea domains and by sequence analysis of dominant members of the community. Operational taxonomic units (OTUs), distinguished as unique ARDRA patterns, showed reproducible distribution for three sample replicates. The highest diversity was observed in the Bacteria domain. The 16S ribosomal DNABacteria clone library contained 75 OTUs, with the dominant OTU accounting for 13% of the total clones, but just 21Archaea OTUs were found, and the most prominent OTU represented 50% of the clones from the respective library. Succession in methanogenic populations was observed, and two periods were distinguished: in the first, Methanobacterium formicicumwas dominant, and in the second, Methanosarcina mazei and aMethanobacterium bryantii-related organism were dominant. Higher variability in Bacteria populations was detected, and the temporal OTU distribution suggested a chaotic pattern. Although dominant OTUs were constantly replaced from one sampling point to the next, phylogenetic analysis indicated that inferred physiologic changes in the community were not as dramatic as were genetic changes. Seven of eight dominant OTUs during the first period clustered with the spirochete group, although a cyclic pattern of substitution occurred among members within this order. A more flexible community structure characterized the second period, since a sequential replacement of aEubacterium-related organism by an unrelated deep-branched organism and finally by a Propionibacterium-like species was observed. Metabolic differences among the dominant fermenters detected suggest that changes in carbon and electron flow occurred during the stable performance and indicate that an extremely dynamic community can maintain a stable ecosystem function.

Objective: To evaluate the safety of in-home use of the MiniMedÔ 670G system with SmartGuardÔ technology in children with type 1 diabetes (T1D). Methods: Participants (N = 105, ages 7-13 years, mean age 10.8-1.8 years) were enrolled at nine centers (eight in the United States and one in Israel) and completed a 2-week baseline run-in phase in Manual Mode followed by a 3-month study phase with Auto Mode enabled. Sensor glucose (SG), glycated hemoglobin (HbA 1c), percentage of SG values across glucose ranges, and SG variability, during the run-in and study phases were compared. Participants underwent frequent sample testing with i-STAT Ò venous reference measurement during a hotel period (6 days/5 nights) to evaluate the system's continuous glucose monitoring performance. Results: Auto Mode was used a median of 81% of the time. From baseline to end of study, overall SG dropped by 6.9-17.2 mg/dL (P < 0.001), HbA 1c decreased from 7.9%-0.8% to 7.5%-0.6% (P < 0.001), percentage of time in target glucose range (70-180 mg/dL) increased from 56.2%-11.4% to 65.0%-7.7% (P < 0.001), and the SG coefficient of variation decreased from 39.6%-5.4% to 38.5%-3.8% (P = 0.009). The percentage of SG values within target glucose range was 68.2%-9.1% and that of i-STAT reference values was 65.6%-17.7%. The percentage of values within 20%/20 of the i-STAT reference was 85.2%. There were no episodes of severe hypoglycemia or diabetic ketoacidosis during the study phase. Conclusion: In-home use of MiniMed 670G system Auto Mode for 3 months by children with T1D, similar to MiniMed 670G system use by adolescents and adults with T1D, was safe and associated with reduced HbA 1c levels and increased time in target glucose range, compared with baseline.

Background: This study evaluated the accuracy and performance of a fourth-generation subcutaneous glucose sensor (Guardian™ Sensor 3) in the abdomen and arm.Methods: Eighty-eight subjects (14–75 years of age, mean ± standard deviation [SD] of 42.0 ± 19.1 years) with type 1 or type 2 diabetes participated in the study. Subjects wore two sensors in the abdomen that were paired with either a MiniMed™ 640G insulin pump, or an iPhone® or iPod® touch® running a glucose monitoring mobile application (Guardian Connect system) and a third sensor in the arm, which was connected to a glucose sensor recorder (GSR). Subjects were also asked to undergo in-clinic visits of 12–14 h on study days 1, 3, and 7 for frequent blood glucose sample testing using a Yellow Springs Instrument (YSI) reference.Results: The overall mean absolute relative difference (MARD ± SD) between abdomen sensor glucose (SG) and YSI reference values was 9.6% ± 9.0% and 9.4% ± 9.8% for the MiniMed 640G insulin pump and Guardian Connect system, respectively; and 8.7% ± 8.0% between arm SG and YSI reference values. The percentage of SG values within 20% agreement of the YSI reference value (for YSI >80 mg/dL) was 90.7% with the MiniMed 640G insulin pump, 91.8% with the Guardian Connect system, and 93.1% for GSR-connected arm sensors. Mean functional sensor life, when calibrating 3–4 times/day, was 145.9 ± 39.3 h for sensors paired with the MiniMed 640G insulin pump, 146.1 ± 41.6 h for sensors paired with the Guardian Connect system, and 147.6 ± 40.4 h for sensors connected to the GSR. Responses to survey questions regarding sensor comfort and ease of use were favorable.Conclusions: The Guardian Sensor 3 glucose sensor, whether located in abdomen or the arm, provided accurate glucose readings when compared with the YSI reference and demonstrated functional life commensurate with the intended 7-day use.: NCT02246582

Contamination of fish in the Great Lakes generated three surveys assessing polychlorinated biphenyls (PCB) serum concentration in Michigan anglers: 1973 to 1974, 1979 to 1982, and 1989 to 1991. This cohort provided 1177 individuals with PCB determinations. In 2000, we conducted telephone interviews with parents on their children's birth characteristics. We estimated the sex odds ratio for parental PCB and dichlorodiphenyl dichloroethene concentrations using generalized estimation equations. We identified 208 offspring, within 101 families, born after 1963, with paternal measurements of both PCB and dichlorodiphenyl dichloroethene. When controlling for maternal exposure and parental dichlorodiphenyl dichloroethene, the sex odds ratio was increased if paternal PCB concentrations exceeded 8.1 micrograms/L (sex ratio, 2.29; 95% confidence interval, 1.11 to 4.74). Thus, paternal exposure was linked to a higher proportion of male offspring. These findings are opposite those reported for the Seveso study and are in accordance with those for dioxin exposure in the American veterans study.