Background. Bacteria is main etiologycal factor of pulpo-periapical disease. Therefore, eliminating bacteria is the important part on root canal treatment. It can be obtained by using irrigation agent that has antibacterial effect and endodontic sterilization agent. Beside of its antibacterial effect, the ideal irrigation agent should has an ability to remove necrotic tissue and less toxic. Propolis is well known as a natural substain that has antibacterial effect and less toxicity. So, it has a potential use as irrigation agent compared with common root canal irrigation agent, sodium hypochloryte. Purpose. The aim of this study was to observed the difference of antibacterial effect between propolis solution and sodium hypochlorite. Method. Nine samples of Streptococcus viridans were prepared and each sample devided into three parts namely group P (propolis solution), group N (sodium hypochlorite), and control group S (saline). Each group was treated with 0,01 cc agent of root canal irrigation, then was stored in incubator 370C for 24 hours. After that, inhibition zone diameter of  each group was observed.Result. The average of inhibition zone diameters in sodium hypochlorite’s group was 23,47 mm; in propolis solution’s group was 18,70 mm. There were significant difference of antibacterial effect between propolis solution and sodium hypochlorite, against Streptococcus viridans.  Conclussion. Antibacterial effect of sodium hypochlorite is higher than propolis solution, but we should consider the potential use of natural agent-propolis solution as root canal irrigation due to its antibacterial effect.

Background: Composite resins restoration is a treatment for tooth structure loss due to pathological conditions. Longevity of composite resins restoration can be affected by surface hardness restoration. Glycerin can increase surface hardness restoration with inhibit bond oxygen and free radicals on polymerization composite resins. Purpose: Analyze the increase surface hardness composite resins restoration after glycerin application before light-curing composite resins. Review(s): Of the six journals included in this literature review, five journals reported significant differences because of the obstacles in the polymerization process of the composite resin when composite contact with light-curing will activate the photoinitiator to produce highly reactive free radicals, free radicals will break the double chain carbon bonds of monomers and form single bonds of free radicals with monomers. Bonding of free radicals with monomers will produce polymeric bonds (degree of conversion) which affects the level of surface hardness of the filling. While one journal noted no significant difference in the surface hardness of composite resin after glycerin application. Conclusion: The use of glycerin before light-curing can increase the surface hardness composite resin restorations.

Background: Resin composite is more advanced today, but the marginal leakage still can not be avoided, for it takes the bonding system. Bonding system aims to provide a layer between a substrate and composite so it has a strong bond . The current latest bonding system is called universal bonding. It can be used with 2 techniques, both self etch and total etch. Universal bonding contains 10MDP so there’s no need for etching and priming. 10MDP results in better adhesion than previous bonding monomers  generation. A total etch technique  require a pre-etching step to remove smear layer so that it formed porous as a retentive sites. Purpose : Distinguish the marginal leakage of flowablebulkfill composite overlays with the application of total etch and self etch bonding systems accompanied by immersion of carbonated beverages . Method: Tooth samples were prepared to a depth of 2mm, then the bonding agent was applied directly to the cavity without etching (self etch), etched before the application of bonding agent (total etch ), directly loaded without pre-etching and bonding (control) . using a flowablebulkfill composite as a material restorative. All of the sample both total etch and self-etch  soaked in Cola drinks for 5 minutes then repeated until 10 treatment each day (one day is assumed to be 12 hours) up to 7 days, followed by immersion in the methylene blue 0.5 % for 24 hours . Sample then splittedbuccolingual direction, leakage was assessed by scoring the edge trough the depth of methylene blue penetration.  Result:  Mann-Whitney Test showed a significant differences (P>0.05 ) each group . Conclusion: Marginal leakage of composite restoration with total etch technique and self etch technique is smaller than the control group, and the marginal leakage of composite restoration with total etch technique is smaller than that of self etch group .

Background: Lactobacillus acidophilus is gram-positive bacteria that produces acids from carbohydrates and causing dental caries. Caries treatment is done by the cavitation of teeth which is preceded by cavity disinfection. The purpose of cavity disinfection is to kill microorganisms and reduce the risk of new carious lesions. Bacterial elimination can be done using chlorhexidine and laser. Chlorhexidine is widely used for cleaning cavities but cannot remove biofilms, tissue debris and has limited elimination of bacteria in the dentinal tubules. Another way to eliminate bacteria is using Photodynamic Therapy (PDT) which consists of photosensitizer and laser. Until now there has not been a single ingredient that is considered to cleanse the cavity thoroughly. There has been no research yet that examine the number of Lactobacillus acidophilus after using chlorhexidine 2%, laser diode (405 nm), and combination of 2% chlorhexidine with laser diode (405 nm). Objective: To compare the decreasing number of living Lactobacillus acidophilus after using chlorhexidine 2%, laser diode (405 nm), and combination of chlorhexidine 2% with laser diode (405 nm). Methods: A total of 24 samples of Lactobacillus acidophilus were divided into 4 groups: (I) chlorhexidine 2%, (II) chlorophyll photosensitizer and 75 seconds irradiation, (III) combination of chlorhexidine2%, chlorophyll photosensitizer, and 75 seconds irradiation. After treatment, the sample was incubated 48 hours and the colony count was calculated for each group. Results of the analysis were carried out by ANOVA and Tukey HSD tests with p <0.05. Results: The average number of group colonies (I) was 35.33 CFU/ml, (II) 16.83 CFU/ml, (III) 9.5 CFU/ml, (IV) 123.33 CFU/ml. Conclusion: The combination of 2% chlorhexidine with diode laser (405 nm) gives the least amount of living Lactobacillus acidophilus bacteria compared with the administration of 2% chlorhexidine and laser diode (405 nm).

Background: Smear layer is an accumulation of organic material, debris, and inorganic material in root canal wall. Irrigant solution must be able to eliminate organic and inorganic materials. Herbal extract such as pine contain saponins that can dissolve organic and inorganic materials. Korea is a country that has used pine in this case red pine as an antibacterial medicament. In Indonesia, there are also green pine species. Purpose: To explain the differences in cleanliness of the root canals after irrigation using extracts of red pine (Pinus densiflora) and green pine (Pinus merkusii). Methods: This study used twenty-seven mandibular premolar teeth which were divided into 3 groups each group consisted of 9 premolar teeth. All samples were prepared using ProTaper for Hand Use and irrigated. Group I was irrigated using 2.5% NaOCl, group II was irrigated with red pine extract, and group III was irrigated using green pine extract. Tooth that has been prepared, were cut vertically into 2 and cut horizontally in 1/3 apical section. The root canal surface was observed using a scanning electron microscope. Photomicrograph was observed by three observers using a score and performed an analysis test. Results: There were no difference between red pine (Pinus densiflora) and green pine (Pinus merkusii) in root canal cleanliness with a significance value of 0.169 (p> 0.05) Conclusion: Red pine extract had the same smear layer cleaning power as green pine extract.