Diabetes is a chronic metabolic disorder, as well as a situation of increased oxidative stress. We examined the distribution of somatostatin in gastric tissues of cinnamon extract treated streptozotocin-induced diabetic rats using the immunohistochemistry technique. A total of 30 male Sprague Dawley rats were used in the study. The rats were assigned to five groups as control, sham, cinnamon, diabetes and diabetes + cinnamon. No application was made to the control group, the sham group received intraperitoneally (i.p.) 50 mg/kg sodium citrate, and diabetes was induced by i.p. injection of 50 mg/kg STZ in diabetes and diabetes + cinnamon groups. Cinnamon extracts were then given to cinnamon and diabetes + cinnamon groups by oral gavage at a dose of 200 mg/kg for 14 days. The streptavidin-biotin-peroxidase method was used to determine the immunoreactivity of somatostatin. Gastric tissue sections were prepared and stained by Crossman's triple and Hematoxylin-Eosin staining in order to examine histological structure of the gastric tissue. We determined that somatostatin immunoreactivity of the control, sham and cinnamon groups was stronger than for the diabetes, and diabetes + cinnamon groups. While a weak immunoreactivity was found in the cardia, fundus and pyloric mucosa of the gastric tissue in the diabetes and diabetes + cinnamon groups, a strong immunoreactivity was found in the cinnamon, sham, and control groups. Also, a statistically significant was observed when all groups compared in terms of count of parietal and principal cells (P<0.001). It was determined that there was a statistically significant difference between diabetes, diabetes + cinnamon groups and control, sham, cinnamon groups in terms of fasting blood glucose levels (P<0.05). In conclusion, somatostatin, which plays an important role in gastroduodenal diseases, was found to be lower in the diabetes and cinnamon + diabetes groups. ÖzDiyabet, kronik metabolik bir bozukluk olduğu gibi aynı zamanda da artmış bir oksidatif stres durumudur. Çalışmamızda immunohistokimyasal teknik kullanarak tarçın uygulanan streptozotosin ile diabet oluşturulan ratların mide dokusundaki somatostatinin salınımını inceledik. Çalışmada 30 adet Sprague Dawley cinsi erkek rat kullanıldı. Deney grupları kontrol, sham, tarçın, diyabet ve diyabet + tarçın olarak 5 gruba ayrıldı. Kontrol grubuna herhangi bir uygulama yapılmadı, sham grubuna intraperitoneal (i.p.) olarak 50 mg/kg sodyum sitrate uygulandı. Diyabet ve diyabet + tarçın gruplarına i.p. 50 mg/kg STZ enjeksiyonu yapılarak diabet oluşturuldu. Tarçın ve diyabet + tarçın gruplarına tarçın ekstraktı 200 mg/kg olacak şekilde oral gavaj yolu ile 14 gün verildi. Somatostatinin immunoreaktivitesini belirlemek için streptavidin-biotin-peroxidase metotu uygulandı. Mide dokularının normal histolojik yapısını incelemek için Crossman'ın üçlü boyama yöntemi ve Hematoksilen-Eosin boyaması uygulandı. Kontrol, sham ve tarçın gruplarındaki somotostatin immunoreaktivitesi, diyabet ve diyabet + tarçın gruplarından daha güçlü olduğu tes...

No abstract

Objective: This study was conducted with the purpose of investigating the distribution of the Activating Transcription Factor 6 (ATF6) and the Nerve Growth Factor (NGF) in the duodenum tissue of diabetic and non-diabetic rats. Material and Method:Eighteen female Sprague dawley rats were randomly divided into three groups as the control, sham and diabetes groups. Routine histological and immunohistochemical methods were applied on the duodenum tissues collected at the end of the study.Results: It was determined that the villus length measurements showed a statistically significant difference between the control and diabetes groups. There was NGF immunoreactivity which was moderate and diffuse cytoplasmic in the villus intestinalis and muscularis layer in all groups, weak in the crypts and glands in the control and sham groups and moderate and diffuse cytoplasmic in the diabetes group. ATF6 immunoreactivity was determined moderate in the villus intestinalis, crypts, glands and muscularis layer in the control and sham groups and strong diffuse cytoplasmic in the diabetes group. Conclusion: It was determined that both NGF and ATF6 immunoreactivity increased in the duodenum tissue of the rats on which diabetes was induced experimentally.

No abstract

This study was conducted to immunohistochemically investigate Trk-A receptor levels in pancreas tissue of mice treated by cumin (Cuminum cyminum) plant essential oil. Mice were grouped into control group (n = 10) and trial group (n = 10). No application was performed to the mice in the control group. The mice in trial group were treated by 500 mg/kg of oral cumin essential oil every 24 hours for two days. At the end of study, the pancreatic tissues obtained were blocked in paraffin following routine histological processes. Triple staining was performed to the sections taken from these blocks to examine general histological structure of pancreas. Acinus, islets of Langerhans, pars initialis, pars excretory and ductus excretorius were determined in mice pancreas. Immunohistochemical studies showed that all mice had Trk-A immunoreactivity in pancreatic tissue. Moderate immunoreactivity in acini and weak immunoreactivity in islets of Langerhans and excretory ducts were detected in pancreas tissue of mice in control and trial groups. It was determined that there was no difference between the groups in terms of Trk-A immunoreactivity in acini and islets of Langerhans. Based on the immunohistochemical results, cumin was used in field of diuretic, degassing, digestion facilitator, antimicrobial and antidiabetic effects in field of traditional medicine; It was concluded that Trk-A receptor synthesized from pancreatic tissue does not change its levels.