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The aim of this study was to determine the content of rutin in Hemidesmus indicus and to optimize the high‐performance thin‐layer chromatography method. The method was validated in compliance with the International Council for Harmonisation guidelines Q2 (R1) for parameters such as linearity, accuracy, precision, robustness, limit of detection, and limit of quantitation. A Box–Behnken design and response surface methodology has been used to investigate the impact of independent variables on the response. Three independent variables, mobile phase composition (% v/v), mobile phase volume (mL), and duration of saturation (min), were studied. Rutin was verified, and its content was determined using a validated high‐performance thin‐layer chromatography method with good linearity within the range of 200–1000 ng spot–1 with r2 = 0.9998 and correlation coefficient with calibration curve equation y = 0.0297x + 0.0001. The average percentage recovery values varied from 99.03 to 101.15 and 98.88 to 100.12%, respectively, for in‐house and marketed mother tincture). The peak area determination at three different concentration levels shows low values of percentage relative standard deviation (<2%) for inter‐day (0.04–0.06) and intra‐day (0.04–0.05) precision of rutin. The average content of rutin in extract and marketed mother tincture was 229 ± 0.57 and 210 ± 0.57 μg g–1. The proposed method was simple, precise, and accurate for the determination of rutin with frequent quality control assessment of H. indicus.

Development of a method is crucial for discovery, development, and analysis of medicines in the pharmaceutical formulation. Method validation could also be thought to be one in all the foremost well-known areas in analytical chemistry as is reproduced within the substantial variety of articles submitted and presented in peer review journals every year. Validation of an analytical procedure is to demonstrate that it's appropriate for its intended purpose. Results from method validation are often wont to decide the quality, reliability and consistency of analytical results. Analytical methods need to be validated or revalidated. This review describes general approach towards validation process and validation parameters to be considered during validation of an analytical method. It also refers to various regulatory requirements like WHO, USFDA, EMEA, ICH, ISO/IEC. The parameters described here are according to ICH guidelines which include accuracy, precision, specificity, limit of detection, limit of quantification, linearity range and robustness.

According to the World Health Organization (WHO), intimate partner violence is one of the main risks to women's health worldwide. Men can also become victims of domestic violence; as well as female victims tend to present their injuries first to the family doctor or the emergency room. Domestic violence against men is thus a current topic for doctors of all specialties. Studies identified by the search provided prevalence rates of domestic physical violence against men ranging from 3.4% to 20.3%. Most affected men were violent towards their partners. 10.6-40% of them reported being abused or mistreated as children. Alcohol abuse, jealousy, mental illness, physical disability, and short relationship duration are associated with a higher risk of becoming a victim of domestic violence. Reported consequences of violence usually include minor physical injuries, impaired physical health, mental health problems such as anxiety or disruptive disorder, and increased use of alcohol and/or illegal drugs.

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