Jewawut has been used by the community as a food ingredient for noodles, pastries, porridge, juice and health drinks. The barley plant is rich in protein which is equivalent to rice. Protein has many functions in our body, as a building block, regulating substance and producing calories. As a body building substance, protein serves to provide material for growth, tissue formation and for tissue maintenance. Analysis of protein content is useful for measuring protein levels in food ingredients. This study aims to determine the water content, crude fiber, carbohydrates and protein content of barley seeds found in the Balanipa District, Polman Regency. The research was carried out at the Makassar Health Center Laboratory January – February 2022, the data obtained were presented in a quantitative descriptive manner by doing three replications. water content is 5.32%, crude fiber is 2.36%, carbohydrates are 77.39% and barley seed protein is 9.47%. The protein and carbohydrate content of barley in Lambanan Village, Balanipa District, Polewali Mandar Regency is equivalent to the protein and carbohydrate content in white rice and brown rice.

Consumption of staple foods of the community at this time depends on rice. Barley belongs to the cereal group which contains carbohydrates and protein which is not inferior to white rice or brown rice so that it has the potential to be applied to the food industry. Isolation of protein with pH variation to precipitate protein at its isoelectric point and observe the effect of pH on protein solubility. The Kjeldahl method is used to indirectly analyze crude protein levels in foodstuffs. Knowing the yield of protein isolate, N total protein before isolation and after isolation and crude fiber of barley seeds found in the Lambanan area, Balanipa District, Polman Regency, West Sulawesi. The research was carried out at the Makassar Health Center Laboratory and Post-Harvest Laboratories, Faculty of Agriculture, Indonesian Christian University, Toraja January – February 2022, the data obtained were presented in a quantitative descriptive manner by doing three replications. The highest protein isolate yield was at pH 12, which was 64.06%, the highest protein isolate content was at pH 11, which was 20.72%, and the crude fiber content of barley seeds was 1.51%.