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Abstrak. Kenikir (Cosmos caudatus) adalah tanaman yang sering dimanfaatkan daunnya untuk bahan pangan maupun juga sebagai obat-obatan karena memiliki kandungan senyawa fenolik yang cukup tinggi. Ekstraksi senyawa fenol menggunakan metode konvensional masih memerlukan waktu yang cukup lama dan jumlah pelarut yang banyak. Sehingga perlu dicari alternatif metode ekstraksi yang memiliki waktu ekstraksi dan kebutuhan pelarut yang relatif rendah seperti menggunakan Microwave Assisted Extraction (MAE). Tujuan dari penelitian ini adalah melakukan ekstraksi senyawa fenolik dari daun kenikir dengan metode MAE terhadap total fenol dan aktivitas antioksidan. Penelitian ini menggunakan 2 faktor perlakuan yaitu rasio pelarut terhadap kenikir segar yaitu 1:4, 1:6, dan 1:8 (b/v) dan waktu ekstraksi 2, 3 dan 4 menit. Penelitian ini menjelaskan bahwa nilai total fenol tertinggi (17,162 ± 0,11 mg GAE/g berat segar) diperoleh pada perlakuan rasio pelarut 1:8 (b/v), sedangkan aktivitas antioksidan tertinggi (4,203 ± 0,26 mg/ml) pada perlakuan rasio pelarut 1:4 (b/v). Waktu perlakuan 3 menit menunjukkan hasil yang optimal untuk nilai total fenol dan aktivitas antioksidan. Hasil tersebut juga menunjukkan nilai yang lebih baik jika dibandingkan dengan metode konvensional baik dengan maserasi biasa dan maserasi dengan waterbath. Extraction of Phenolic Compounds from Cosmos caudatus using Microwave Assisted Extraction (MAE) Abstract. Cosmos caudatus, a plant commonly used its leaves for food and also as a medicine since it contains high phenolic compounds. The extraction of phenolic compounds using conventional methods still require more extraction time and need much more of solvent. Therefore, it was necessary to find an alternative extraction method that has a rapid extraction time and a few solvent need such as using Microwave Assisted Extraction (MAE). The aim of this study is to extract of phenolic compounds from Cosmos caudatus with MAE method of total phenol and antioxidant activity. This study using 2 treatment factors, the sampel-solvent ratio of 1:4, 1:6, and 1:8 (w/v) and the extraction time of 2, 3 and 4 minutes. This study explained that the highest total phenolic compounds (17.162 ± 0.11 mg GAE/g fw) and antioxidant activity (4.203 ± 0.26 mg/ml) were obtained at 1:8 and 1:4 (w/v) of sampel-solvent ratio, respectively. The extraction time of 3 minutes was the optimal condition for total phenolic compounds and antioxidant activity. These results also showed the highest parameter value when compared with both conventional methods: maceration and maceration with waterbath.

Pineapple which is one of the perishable commodities with high water content about 90.73% in 100 gr of fresh material. Thermal pasteurization has a weakness that can degrade the quality of fruit juice products. In contrast, non thermal processes have expectations which is don’t make decreasing quality of the product. One of the non-thermal pasteurization type called Pulsed Electric Field (PEF). Nowadays, PEF system have two type batch and continous, which respectively used in this research is PEF continous system. This study uses research methods group random design (GRD) with two factorial that voltage (20, 30 and 40 kV) and frequency (10, 20, 30 and 40 kHz). Based on these studies it is known that the voltage and frequency is significantly different to the value of Total Plate Count (TPC), and absorbance. However there aren’t significantly different with the pH, vitamin C, viscosity, total dissolved solid (TDS), total sugar and color. The treatment based on the microbial test are at a voltage of 40 kV and a frequency of 40 kHz which can reduce bacteria significantly 1.01x103 cfu/mL or 0.94 by log cycle with the effectiveness of microbial death by 88.5%. The treatment based on the microbial test are at a voltage of 40 kV and a frequency of 30 kHz which The result indicates that physical and chemical characteristic of pineapple juice become 3.98 of pH, total dissolved solid (TDS) of 6.25 °brix, total sugar of 14.75 % viscosity of 3 cp, vitamin C of 7.6 mg/100ml, absorbance of 0.67, color on the brightness (L*) value of 24.05, redness (a*) of 6.35, and yellowish (b*) of 7.5. This result shows that PEF continuous respectively work system to pasteurize the pineapple juice.

The application of plant acoustic frequency technology (PAFT) can spur plant growth, increase productivity, immune system, quality, and extend the shelf life of agricultural products after harvest. The application of sound waves in plants can stimulate the opening of the stomata to optimally absorb nutrients and water. This study aims to determine the effect of frequency and time of PAFT exposure by utilizing Javanese gamelan traditional music on the vegetative growth of red-lettuce (Lactuca sativa var.). Javanese gamelan music used was titled Puspawarna with variations in the frequency of 3-5, 7-9, and 11-13 kHz. The variation of exposure time of sound waves was 1, 2, and 3 hours. PAFT exposure was given routinely in the morning and evening. The results of this study indicated that the best treatment was at 3-5 kHz with an exposure time of 2 hours. This treatment gave a significantly better effect when compared to plants without PAFT. The best combination of frequency and time of PAFT exposure produced 10 leaves, plant height of 9.4 cm, wet weight of 4 g, dry weight of 0.22 g, leaf area of 27.19 cm 2 , leaf red mean of 63, and stomata opening width of 145.44~206.59 µm.

The objective of this research was to isolate, screen, and identify the cellulase and xylanase-producing mold from oil palm waste. There are thirty-two isolates from oil palm waste which are able to degrade and grow on media containing cellulose or xylan as a sole carbon source. Screening to determine cellulolytic and xylanolytic activity was performed by paper disc diffusion method using Congo Red as an indicator. All the thirty-two mold isolates showed cellulolytic and xylanolytic activity with relative enzyme activity (ratio of hydrolysed zone diameter and diameter of colony growth) ranging from 1.04 to 1.62. Based on the macro- and micromorphology characteristics, these isolates were identified as genus Trichoderma, Aspergillus, Rhizopus, Tallaromyces and Penicillium, with the number isolates in each genus was 22, 4, 3, 2, and 1 respectively. The highest cellulolytic and xylanolytic activity was achieved from the isolate namely OPT1(4) which was identified as Talaromyces pinophilus using morphological and molecular analysis. Under the optimum condition (30°C, 72 hours) in liquid medium containing carboxymethyl cellulose (CMC) and birch wood xylan as the main carbon source, Talaromyces pinophilus OPT1(4) presented glucanase and xylanase activity of 15.17 U/mL and 13.35 U/mL, respectively.