Objectives: This study was conducted to investigate the effect of health beliefs, safety motivations, and safety-climate on the performance of taking standard precautions of nursing college students with clinical practice experience. Methods: The subjects of this study were 173 students in the 3rd and 4th grades of nursing college located in Daegu Metropolitan City with clinical practice experience, and data were collected from September 7th to September 25th, 2020 using a structured questionnaire. Results: As a result of the study, the health belief of nursing college students was 3.60 (± 0.75) points, safety motivation 22.40 (± 5.33) points, safety-climate 4.79 (± 1.90) points, and the performance of taking standard precautions was 3.21 (± 0.63) points. Hierarchical regression analysis was performed with the presence or absence of infection management education, health belief, safety motivation, and safety-climate as independent variables to identify the factors that affect the performance of taking standard precautions. Factors affecting the performance of taking standard precautions are health belief (β = 0.33, p = 0.003), safety motivation (β = 0.28, p = 0.003), safety-climate (β = 0.20, p = 0.009). Conclusions: According to this study, health belief, safety motivation, and safety-climate influenced the performance of taking standard precautions. These results suggest that it is necessary to emphasize the importance of practicing standardism, motivate nursing students, and provide a safer hospital practice environment in order to improve the performance of taking standard precaution when nursing college students are in hospital practice.