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Cyperus rotundus (Musta), is a medicinal plant growing in tropical, sub-tropical and temperate regions having many pharmacological and medicinal characteristics. The current study attempts to standardize the extracts of raw drug Musta as per pharmacopoeial requirement. Roots and rhizomes of Musta sold in the markets of Mangalore were collected. Test sample was analysed for ash and extractive values. Tests for phytochemicals like alkaloids, flavonoids, steroids, phenols, carbohydrates, saponins, tannins and coumarins were performed in both ethanolic and aqueous extracts. Total phenolic content of the extracts were done as per standard protocol. Fingerprint profile of ethanolic and aqueous extract has been derived by photodocumentation and HPTLC densitometric scan. Ash values, extractive values, phytochemical tests, total phenol content and the HPTLC fingerprint of the Musta has been derived from the current study. The set of values obtained from the studies can be used as standards for testing, standardization and quality control of medicinal materials sold as Musta.

Standardization of Ayurvedic formulations is an important step for establishment of biological activity, consistent chemical profile, or quality assurance for production and manufacturing of herbal drugs. Most of the pharmaceutical industries are using substitute drugs instead of authentic drugs. Karshyahara Yoga (KY) is an Ayurvedic polyherbal nutritive drug comprising of Vidarikanda (Pueraria tuberose (Willd.) DC.) -1 part, Godhuma (Triticum sativum L.) -1 part, Yava (Hordeum vulgare L.) -1 part, Sita (sugar) -2 parts and Pippali (Piper longum L.) -1/20 th part possessing bhrumhana (nourishing) action. Aims of the study was to standardize Karshyahara Yoga (KY), employing standard testing protocol for AYUSH drugs. Physico----chemical, proximate and chemical fingerprint studies like loss on drying at 105 o C, total ash, acid insoluble ash, water soluble ash, alcohol soluble extractive, water soluble extractive, total fat, crude fibre, total carbohydrate, total protein and HPTLC performed as per standard methodology. Quality indicating physical and chemical tests was done and standard values for KY were recorded. Standardization tests done on KY helped in authenticating the polyherbal preparation and also in ensuring the quality of the same.

Medicinal herbs are to be harvested during their optimal season to obtain herbal products of the best possible quality. In the criteria for standardization, season of collection of the used part of a plant plays an important role to assure the quality of the drug. Ayurvedic classics have advocated certain season for the collection of used parts of the medicinal plants. Haridra kanda (rhizome of Curcuma longa Linn.) has wide range of applications as antioxidant, antiinflammatory, anti diabetic, anticarcinogenic, hypocholesterolemic, wound healing, antitumor and hepatoprotective agent. It is one of the important drug in Ayurveda used therapeutically in charmadosa, prameha, raktavikara, sotha, pandu, vrana etc. This study was carried out to evaluate the effect of sangraha kala (seasons of collection) of Haridra on quality indicating Pharmacopoeial constants. Haridra was cultivated and collected at different seasons and the procured rhizomes were powdered and subjected to qualitative and quantitative analysis. Macroscopic, microscopic, physicochemical, solvent extraction and chromatographic studies carried out suggest that Haridra collected during Sharad rutu (autumn season) contain more active principles to meet Pharmacopoeial requirements. It may be concluded that Sharad rutu is the ideal season for collection of Haridra rhizomes, which revalidates the classical reference in ancient Ayurvedic texts.