We present polarization-sensitive Fourier ptychographic microscopy (PS-FPM) capable of generating high-resolution birefringence images of optically anisotropic specimens over a large field of view (FoV). FPM produces high-resolution images of transparent samples over a large FoV based on multiple intensity measurements acquired at various illumination angles and ptychographic phase retrieval. We combine this attractive feature of FPM with a single-input-state illumination and polarizationdiverse imaging system to achieve the imaging of both complex and birefringence information on transparent objects. Compared to conventional polarization imaging techniques, PS-FPM does not involve any mechanical rotation of the polarizer/analyzer and achieves birefringence imaging with a half-pitch resolution of 0.55 μm over 3.78 mm 2 FoV, which corresponds to the spacebandwidth product of 12.5 megapixels. We demonstrate the high-resolution, large-area birefringence imaging capability of PS-FPM by presenting the birefringence images of various anisotropic objects including monosodium urate, Tilia stem, and hemozoin crystals.
We present a novel, to the best of our knowledge, form of polarization microscopy capable of producing quantitative optic-axis and phase retardation maps of transparent and anisotropic materials. The proposed method operates on differential phase-contrast (DPC) microscopy that produces a phase image of a thin specimen using multi-axis intensity measurements. For polarization-sensitive imaging, patterned illumination light is circularly polarized to illuminate a specimen. The light transmitted through a specimen is split into two orthogonal polarization states and measured by an image sensor. Subsequent DPC computation based on the illumination patterns, acquired images, and the imaging model enables the retrieval of polarization-dependent quantitative phase images, which are utilized to reconstruct the orientation and retardation of the specimen. We demonstrate the validity of the proposed method by measuring the optic-axis and phase retardation maps of calibrated and various anisotropic samples.
ReMember is an interactive installation that provides bereaved pet owners with a sense of presence and connection to their deceased companion animals. Many pet owners suffer from bereavement and grief upon the loss of companion animals, and rituals and memorialization can help their grieving process. In this installation, the authors integrate audiovisual effects of the heartbeat recordings of companion animals and cremation stones made for pet memorialization. The audience's responses to ReMember show that interactivity and physiological signals augment the physical object of remembrance and elicit strong emotions. ReMember suggests that digitalized physiological signals and biological data can be a new form of the legacy of animal companionships and that the careful curation of digital, biological, and physical artifacts can support continuing bond expressions in pet loss.
We present polarization-sensitive Fourier ptychographic microscopy (PS-FPM) capable of generating high-resolution birefringence images of optically anisotropic specimens with large field-of-view (FoV). FPM produces high-resolution complex object field of transparent samples over a large-FoV based on multiple intensity measurements acquired with various illumination angles and ptychographic iteration engine. We combine this attractive feature of FPM with single-input-state illumination and pixelated polarized camera to achieve imaging of both complex and birefringence information of transparent objects. Compared to conventional polarization imaging techniques, PS-FPM does not involve any mechanical rotation of polarizer/analyzer and achieves birefringence imaging with a half-pitch resolution of 0.55-μm over 3.78-mm2 FoV, which corresponds to the space-bandwidth product of 12.5 megapixels. We demonstrate high-resolution, large-area birefringence imaging capability of PS-FPM by presenting birefringence images of various anisotropic objects.
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