Objectives Inhaled corticosteroids are the most effective controllers of asthma, although asthmatics vary in their response. FKBP51 is a major component of the glucocorticoid receptor which regulates its responses to corticosteroids. Therefore, the present study aims to identify the role of FKBP5 gene polymorphism in asthma susceptibility and corticosteroid resistance. Methods DNA was extracted from the blood of 68 asthmatic and 40 control subjects. FKBP5 gene fragments were amplified by PCR and sequenced by the Sanger method. The sequencing results were aligned by mapping on the reference sequences of National center of Biotechnology Information (NCBI) and single nucleotide polymorphisms (SNPs) which were checked. Finally, the genotype, allele frequency and odds ratio (OR) were calculated. Results The FKBP5 fragment sequencing revealed the presence of rs1360780 and one novel SNP found in 17 samples taken from asthmatic patients as compared to db SNP data in the NCBI database. The FKBP5 variant (rs1360780) indicated that the allele frequency of risk allele T was 41.18% in patients and 20% in control group members p<0.001 and OR=2.8 when compared to a wild C allele frequency of 58.82% in patients and 64% in the control group members. The novel SNP FKBP5 was compared to the SNP database in the NCBI database in which wild T allele was substituted with G. The novel SNP was submitted to the ClinVar Submission Portal at NCBI with accession number: rs1581842283 and confirmed an asthma susceptibility risk factor with allele G frequency of 11.76% in asthmatics and 2.5% in the control group members (OR=5.2, p<0.05), as compared to a wild T allele frequency of 88.24% in asthmatics and 97.5% in the control group members. Conclusions The risk allele T of rs1360780 and the novel SNP rs1581842283 risk allele G predict asthma susceptibility but show no association with corticosteroid resistant.

The aim of the study was to investigate the effect of magnetized water on the histological structure of heart, lung and spleen. For this purpose, twenty five albino rats were divided into five equal groups, the first group was considered as control group. The other groups were given magnetized water with intensity of 250, 750, 1000, 1500 gause every day for 30 days. Then the animals were sacrificed and the histological change on heart, lung and spleen was studied. Histopathology of heart in rats treated with magnetic water with intensity of 250, 750, 1000, 1500 gause showed no clear pathological lesion. Lung section of rats treated with 250 gause of magnetic water showed no pathological lesion, while lung section belongs to rats group given magnetic water with intensity of 750, 1000 gause showed hyperplasia of lymphoid tissue in the wall of bronchiole and thickening to the wall alveoli, in addition the lung section belongs to magnetic water with intensity of 1500 agues treated rats showed thickening in alveolar wall. Spleen tissue belongs to magnetic water with intensity of 250 gause treated rats showed hyperplasia of the white pulp, while spleen tissue belongs to magnetic water with intensity of 750 gause treated rats showed a marked hyperplasia of the lymphoid tissue in the periarterial sheath. Also, spleen tissue belongs to magnetic water with intensity of 1000 gause treated rats showed amyloid like substance deposition around the white pulp. Necrotic area of lymphoid tissue was observed in the spleen tissue belongs to rat groups given magnetic water with intensity of 1500 gause.

Polycystic ovary syndrome (PCOS) is reproductive, endocrine and metabolic disorder. The pathology of PCOS is complicated and associated to chronic low-grade inflammation. Which associated with high level of pro-inflammatory cytokines, chemokines and leukocyte count. Inflammation of the ovary effects on ovulation and induce or aggravates systemic inflammation. MIP-1 proteins are tiny (8-10 kDa), pro-inflammatory chemokines of the CC subfamily, one of four chemokine families identified by their primary structure (i.e., CXC, C, CC, and CX3C). T- and B-lymphocytes, neutrophils, Macrophages, mast cells, dendritic cells and natural killer cells are all capable of producing large amounts of MIP-1. Materials and Methods: The research was performed on two groups, from 60 PCOS women and 30 control women during the period from October 2022 to January 2023. The diagnosis of PCOS women was based on two out of three of the following diagnostic criteria (hyperandrogenism - oligo or anovulation - polycystic ovaries). MIP-1 alpha and Beta levels were detected by ELISA. Results: The outcomes revealed that 60 women with PCOS showed significant increase (P<0.05) in the level of MIP1 alpha (635.28 ±20.58) while in women without PCOS was (571.20 ±25.92) , as well as non-significant increase the level of MIP1 beta in women with PCOS (191.85 ±17.54) while in control group (165.31 ±11.01). Conclusion: The current study concluded that MIP1 alpha and MIP1 beta increase, this prove that PCOS is low grade chronic inflammation.

Asthma is a chronic inflammatory disease affecting 5% of the world population. FKBP51 is an important immunophilin modular protein of the glucocorticoid receptor (GC). The aim of the present study was to evaluate the levels and immunocytochemical distribution of FKBP51 and GR in lymphocyte cells of asthmatic patients, by use of immunocytochemistry method, and to assesslevels ofstress hormones (cortisol and ACTH) by radioimmuniassay (RIA). The results showed significantly increased nuclear localization and decreasedcytoplasmic distribution of FKBP51, while they showed a significant increase in nuclear localization and a non-significant decrease in cytoplasmic distribution of GRin asthmatic patients(P<0.05). Cortisol and ACTH levels were also measured and showedinsignificant increases(P<0.05)in steroid treated (338.85 ±139.5 mMol/L, 35.05±3.77 ng/ml, respectively)and non steroid treated asthmatics(280.5 ± 74.6 mMol/L, 32.0±6.43 ng/ml, respectively)as compared with the control group (234.33±29.13 mMol/L, 29.0±7.02 ng/ml, respectively).

This study aimed to investigate the histopathological changes of Indomy pasta for 30 days on lung, kidney and spleen of albino rats. fifteen adult albino rats 200-220 gm was divided into 3 groups: The first group was considered as control group. The second group was fed with Indomy every other day for 30 days; the third group was fed with Indomy every day for 30 days. The histopathogical examination of the kidney show congestion of blood vessels and infiltration with neutrophil in lumen, The histopathological examination of lung showed hyper atrophy of masculares layer of the bronchiole with sever inflammatory cell infiltration mainly neutrophil and mononuclear cell in the wall of bronchioles, and with lumen of the alveoli with fibrous connective tissue proliferation in the parenchyma. Also, increase thickness into intra alveolar septa due to paranchymal cell proliferation and mononuclear cell infiltration which lead to narrow alveolar lumen. The histopathogical examination of spleen shows congestion of the red pulp. From the results of this study it can be conclude that the consumption of Indomy had harmful histopathological effects on kidney, lung and spleen tissues in treated rats.