Cassia species have been of medicinal interest due to their good therapeutic value in folk medicine. In the present study petroleum ether, ethanol and chloroform extracts from leaf and stems of Cassia obtusifolia and Senna sophera were investigated for their antimicrobial activities against some pathogenic microbes in vitro. The C. Obtusifolia leaf extracts in pet ether and chloroform showed more sensitivity against E. faecalis (MIC 0.2725mg/ml and MIC 0.2647) and ethanol extracts against A. fumigatus (MIC 0.3116mg/ml). Similarly the stem extracts of C. Obtusifolia in pet ether showed more sensitivity against E. faecalis (MIC 0.407mg/ml), ethanol extracts against E. faecalis (MIC 0.3009mg/ml) and chloroform extracts against E. faecalis MIC 0.4946mg/ml). The leaf extracts of S. sophera in pet ether showed more sensitivity against C. albicans (MIC 0.3524mg/ml), ethanol extracts against E. faecalis (MIC 0.2738mg/ml) and chloroform extracts against C. Albicans (MIC 0.4239). C. sophera stem extracts in Pet ether showed more sensitivity against E. faecalis (MIC 0.254mg/ml), ethanol extracts against E. faecalis (MIC 0.2987mg/ml) and chloroform extracts against E. faecalis (MIC 0.5899mg/ml). This finding provides an insight into the usage of the leaves of Cassia species in traditional treatment of wounds or burns associated with bacterial and fungal infections. However, further work is needed in the form of phytochemical screening and pharmacological activity of some more extracts before one could conclude anything definite about the therapeutic potential of these extracts.

Objective: The use of plants for medicinal remedies is an integral part of the Indian cultural life, and the traditional background of Indian medicine shows widespread use of plant products in cancer treatment. In this study, stem extracts of some selected Cassia species have been evaluated for their cytotoxic activities under in vitro conditions. Methods:The stems were shade dried at room temperature. The dried and coarsely powdered plant material were extracted with petroleum ether (60-80°C), chloroform, and ethanol using soxhlet apparatus. The cytotoxicity was evaluated by [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay.Results: Stem extracts of three Cassia species, viz., Cassia glauca, Cassia Obtusifolia, and Cassia sophera have been evaluated for their cytotoxic activities with chloroform, ethanol and pet ether against HeLa and breast cancer cell lines. Among the three different solvents used at different concentrations, the chloroform extracts of all Cassia species exhibited maximum cytotoxicity (%) against both cell lines. The CTC 50 values are revealed the cytotoxic potential of C. glauca chloroform extracts against HeLa cell line and breast cancer cell lines with CTC 50 values 180.00±3.0 and 146.67±0.5, respectively. Chloroform extracts of C. Obtusifolia and C. sophera showed maximum activity against HeLa (380.00±1.1 and 800.00±1.7, respectively) and breast cancer cell lines (310.00±1.1 and 633.33±0.6). Conclusion:The results of this study demonstrate the potent cytotoxic activity of chloroform extracts of stems of Cassia species against HeLa and Breast cancer cell lines.